Detailed information NTG0343795

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: EPB58_RS19000
• Genome accession: NZ_CP035693
• Coordinates: 1884402..1885271 (-)
• Length: 289 a.a.
• NCBI ID: WP_005446255.1
• Uniprot ID: A0A3A1Q6Q3
• Organism: Vibrio harveyi strain 2011V-1164
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1879402..1890271

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EPB58_RS18965	rpsP	1880205..1880453 (+)	249	WP_004410028.1	30S ribosomal protein S16	-
EPB58_RS18970	rimM	1880482..1881030 (+)	549	WP_005446245.1	ribosome maturation factor RimM	-
EPB58_RS18975	trmD	1881058..1881801 (+)	744	WP_005446247.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
EPB58_RS18980	rplS	1881843..1882196 (+)	354	WP_005379971.1	50S ribosomal protein L19	-
EPB58_RS18985	yacG	1882762..1882962 (-)	201	WP_026000212.1	DNA gyrase inhibitor YacG	-
EPB58_RS18990	zapD	1883019..1883759 (-)	741	WP_038897986.1	cell division protein ZapD	-
EPB58_RS18995	coaE	1883787..1884401 (-)	615	WP_154203413.1	dephospho-CoA kinase	-
EPB58_RS19000	pilD	1884402..1885271 (-)	870	WP_005446255.1	A24 family peptidase	Machinery gene
EPB58_RS19005	pilC	1885344..1886567 (-)	1224	WP_154203414.1	type II secretion system F family protein	Machinery gene
EPB58_RS19010	pilB	1886601..1888286 (-)	1686	WP_005446257.1	type IV-A pilus assembly ATPase PilB	Machinery gene
EPB58_RS19015	pilA	1888286..1888690 (-)	405	WP_050936251.1	pilin	Machinery gene
EPB58_RS19020	nadC	1888955..1889842 (-)	888	WP_154203415.1	carboxylating nicotinate-nucleotide diphosphorylase	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32536.74 Da
• Isoelectric Point: 7.5422

>NTDB_id=343795 EPB58_RS19000 WP_005446255.1 1884402..1885271(-) (pilD) [Vibrio harveyi strain 2011V-1164]
MEVFQYYPWLFVVFTAIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIEPPKETLTLSVPRSSCQQCGTHIRIR
DNIPVISWLLLKGKCHNCQSPISARYPLIELLTAFCSGFIAFHFGFSYFTVALVFFTFVLIAATFIDLDTMLLPDQLTLP
LMWAGIALALAGISPVSLQDSIIGAMAGYLCLWSVYWLFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGV
IFGLIQLRLQKQGIEKAFPFGPYLAIAGWVSLIWGNQILNWYFTSILGV

Nucleotide

• Length: 870 bp

>NTDB_id=343795 EPB58_RS19000 WP_005446255.1 1884402..1885271(-) (pilD) [Vibrio harveyi strain 2011V-1164]
ATGGAAGTATTCCAGTACTACCCATGGCTATTCGTTGTATTTACCGCTATTTTTGGTTTGATCGTGGGTAGCTTCCTCAA
CGTAGTTATTTACCGTTTACCTAAGATCATGGAACTAGAATGGCGACGCGAGTGCGCTGAATCATTCCCTGAATACAAGA
TCGAGCCACCAAAAGAGACATTAACCTTAAGCGTGCCACGTTCATCTTGTCAGCAATGCGGTACACATATCCGCATTAGA
GACAATATCCCTGTGATTAGTTGGCTATTACTAAAAGGTAAATGCCACAACTGCCAATCACCTATCAGTGCCCGTTACCC
TCTTATCGAACTGCTTACCGCTTTTTGTTCGGGTTTTATCGCTTTTCATTTCGGTTTTAGCTACTTCACTGTGGCGCTGG
TTTTCTTTACCTTTGTATTGATTGCTGCCACATTTATCGACCTTGATACCATGTTGCTGCCAGACCAACTCACACTACCA
TTGATGTGGGCGGGTATTGCTCTTGCTCTTGCCGGTATCAGCCCTGTGAGTCTTCAAGATTCTATTATTGGAGCAATGGC
AGGCTACTTATGTCTTTGGAGTGTTTATTGGTTATTCAAACTCCTCACAGGCAAAGAGGGAATGGGCTATGGCGACTTTA
AACTCCTTGCTGCATTAGGAGCTTGGCTGGGCTGGCAATCTCTGCCGATGATCATTTTACTTTCTTCGGTAGTAGGCGTC
ATTTTTGGTCTTATCCAGCTGCGCTTGCAAAAGCAAGGTATCGAAAAAGCCTTTCCTTTTGGTCCTTACCTTGCAATTGC
AGGATGGGTGAGCCTAATTTGGGGCAATCAAATCCTCAACTGGTACTTTACGTCGATTCTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A3A1Q6Q3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	97.232	100	0.972
pilD	Vibrio cholerae strain A1552	74.306	99.654	0.74
pilD	Acinetobacter baumannii D1279779	52.768	93.772	0.495
pilD	Acinetobacter nosocomialis M2	52.399	93.772	0.491
pilD	Neisseria gonorrhoeae MS11	45.652	95.502	0.436