Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   EWA65_RS06860 Genome accession   NZ_CP035688
Coordinates   1535118..1537361 (-) Length   747 a.a.
NCBI ID   WP_055027985.1    Uniprot ID   A0A0Q0NB28
Organism   Vibrio metoecus strain 08-2459     
Function   ssDNA transport through the inner membrane (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1530118..1542361
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWA65_RS06840 kdsB 1531411..1532169 (-) 759 WP_055027984.1 3-deoxy-manno-octulosonate cytidylyltransferase -
  EWA65_RS06845 - 1532169..1532348 (-) 180 WP_000350067.1 Trm112 family protein -
  EWA65_RS06850 lpxK 1532329..1533336 (-) 1008 WP_055027995.1 tetraacyldisaccharide 4'-kinase -
  EWA65_RS06855 msbA 1533339..1535087 (-) 1749 WP_154138878.1 lipid A ABC transporter ATP-binding protein/permease MsbA -
  EWA65_RS06860 comEC 1535118..1537361 (-) 2244 WP_055027985.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  EWA65_RS06865 - 1537370..1537900 (+) 531 WP_009355280.1 DUF2062 domain-containing protein -
  EWA65_RS06870 lolE 1538055..1539299 (-) 1245 WP_000493002.1 lipoprotein-releasing ABC transporter permease subunit LolE -
  EWA65_RS06875 lolD 1539300..1539986 (-) 687 WP_055027987.1 lipoprotein-releasing ABC transporter ATP-binding protein LolD -
  EWA65_RS06880 lolC 1539979..1541187 (-) 1209 WP_055044425.1 lipoprotein-releasing ABC transporter permease subunit LolC -
  EWA65_RS06885 - 1541363..1541938 (+) 576 WP_055044424.1 hypothetical protein -

Sequence

Protein


Download         Length: 747 a.a.        Molecular weight: 84741.13 Da        Isoelectric Point: 8.7449

>NTDB_id=343695 EWA65_RS06860 WP_055027985.1 1535118..1537361(-) (comEC) [Vibrio metoecus strain 08-2459]
MTLLSNYWSLISFSITLLSAPYWPWMPTWGWAWFCLILIGVLSYYRACRKLLGFALAIFTVVCHGNLMRDQSNMLSQAGS
DSIIKGQVDSFFTQTRYAYEGLVLIHEVNGQTLNRLMWPRVRLTAPVALKPNEQFEFTVVFKPIVGRLNETGFDLEAHSL
TQSVLARAVVSPNTTYQIVPQHSLRSALLTQLELLTQNSPYQGLILALTFGERKGIGEQEWQALRNSGLIHLVAISGLHI
GIAFSIGYLLGKGLMRLHGKLLWSPFVVGALLAVLYAWLAGFTIPTQRALIMCLLNVGLVILGFPLSALKRILLTLCAVL
VWSPFASLSNSFWMSFLAVAIVLYQLANQEKRQAWWKALLWAQLTLVCLMAPVTAYFFGGLSMTSVVYNLLFIPWFSVVI
VPALFVGLFLSVTMSYWATLYWPCVDWTFYPLDWALQFADVGWVVIPKTVQGFVAALVVISLLYRVMSLRACILAWCLAV
LWWWFPSVTPRWRMDVLDVGHGLAIVIEQDKRAIIYDTGSSWSGGSYVESLIEPILQQRGIRRLDGLILSHLDNDHAGDW
QSLAKRWQPEWIRASQVDVAFMPCMRGERWQWQSLDFQVLWPPRLVNRAYNQHSCVIRMTDLQSNHSVLLSGDVTAMGEW
LLARGGALLSSEVMIVPHHGSKTSSTAEFIAKVEPRMAIASLAKDNRWNLPNPQVVERYQSQQSDWLDTGHAGQISLFFY
PDQFDWFTQRGVGWVPWYRQMLRKGVE

Nucleotide


Download         Length: 2244 bp        

>NTDB_id=343695 EWA65_RS06860 WP_055027985.1 1535118..1537361(-) (comEC) [Vibrio metoecus strain 08-2459]
ATGACTCTCTTGTCGAATTACTGGTCGCTTATCTCTTTTTCGATCACCTTGCTTTCTGCACCGTATTGGCCTTGGATGCC
GACATGGGGCTGGGCTTGGTTTTGCCTTATCCTGATTGGCGTGCTCAGTTATTACCGAGCTTGCCGCAAACTCCTTGGCT
TCGCGTTGGCCATATTCACTGTCGTGTGCCATGGCAACCTAATGCGAGATCAATCCAACATGCTCTCTCAAGCAGGCTCG
GATAGTATCATAAAAGGTCAGGTTGACAGCTTTTTTACGCAAACTCGTTACGCATATGAGGGTTTAGTCTTAATTCATGA
GGTGAATGGTCAAACCTTAAACAGACTGATGTGGCCTCGTGTACGCTTAACGGCTCCAGTAGCGCTAAAACCGAATGAGC
AGTTCGAATTTACGGTTGTATTTAAGCCCATTGTGGGGCGTCTTAATGAAACTGGCTTTGATTTAGAAGCCCACTCTCTG
ACTCAATCGGTTCTTGCGCGAGCGGTTGTCAGCCCGAATACGACATACCAGATTGTGCCGCAACATAGCCTACGCTCCGC
TTTACTGACGCAGCTTGAGTTACTCACCCAAAACAGCCCTTATCAAGGGTTGATATTAGCGCTGACGTTTGGTGAAAGAA
AAGGGATTGGTGAACAAGAATGGCAAGCCTTGCGCAACAGCGGCTTAATTCACTTGGTGGCGATTTCTGGCTTGCATATT
GGTATCGCTTTCAGTATTGGGTACTTGTTAGGCAAGGGGCTGATGCGTCTGCATGGCAAATTGTTGTGGTCTCCTTTCGT
GGTGGGTGCATTATTAGCCGTGTTATACGCATGGCTCGCGGGGTTCACTATACCAACCCAGCGTGCGCTGATTATGTGCT
TGCTGAATGTGGGATTGGTGATCTTGGGGTTTCCTCTTTCAGCATTAAAACGGATCCTGCTGACGTTGTGTGCGGTTTTA
GTTTGGTCGCCCTTTGCATCGCTTTCAAACAGTTTTTGGATGTCATTTCTCGCCGTTGCGATTGTGTTGTATCAATTGGC
CAATCAAGAGAAGCGTCAGGCTTGGTGGAAAGCGTTATTGTGGGCGCAACTGACTCTTGTCTGCTTAATGGCGCCAGTCA
CCGCATACTTTTTCGGTGGATTAAGCATGACGTCCGTAGTGTACAATTTGCTGTTTATCCCTTGGTTCTCCGTGGTGATT
GTTCCCGCTTTGTTTGTGGGTTTGTTTCTCAGCGTGACGATGTCGTATTGGGCGACGCTTTATTGGCCTTGCGTGGACTG
GACGTTCTATCCCCTCGATTGGGCGCTGCAGTTTGCGGATGTAGGATGGGTGGTGATACCGAAAACCGTACAAGGTTTCG
TGGCTGCCTTAGTCGTGATCAGTTTGTTGTACCGAGTGATGAGCTTACGAGCTTGCATTTTGGCATGGTGTCTTGCGGTG
CTGTGGTGGTGGTTTCCTTCTGTCACGCCTCGGTGGCGCATGGATGTTTTGGATGTCGGGCACGGTTTAGCCATCGTGAT
TGAGCAAGATAAGCGCGCCATTATCTATGATACGGGGAGCAGTTGGTCGGGAGGCAGTTATGTAGAAAGTCTGATCGAGC
CCATTTTGCAACAGCGGGGTATTCGCCGGCTCGATGGATTGATTCTAAGTCACCTCGATAATGATCATGCCGGAGATTGG
CAAAGTTTGGCCAAGCGTTGGCAACCGGAATGGATACGTGCAAGCCAAGTTGATGTGGCGTTTATGCCCTGTATGCGCGG
TGAGCGCTGGCAATGGCAGTCATTAGATTTCCAAGTGCTATGGCCTCCGAGGCTGGTGAATCGAGCCTACAATCAGCATT
CGTGTGTGATTCGCATGACCGATCTCCAATCTAACCATTCCGTGTTGTTATCTGGGGATGTCACCGCCATGGGAGAATGG
CTACTTGCTCGTGGAGGTGCGCTTTTATCGAGCGAAGTGATGATAGTGCCTCATCACGGCAGCAAAACCTCATCCACCGC
CGAGTTTATTGCGAAAGTCGAACCACGGATGGCGATTGCTTCCCTCGCCAAAGATAACCGCTGGAATTTGCCTAATCCAC
AAGTAGTGGAGCGTTATCAAAGCCAGCAATCTGATTGGCTAGATACTGGCCACGCGGGACAAATCAGTCTGTTTTTCTAT
CCAGATCAGTTTGATTGGTTTACTCAGCGTGGAGTTGGCTGGGTGCCATGGTATAGGCAGATGCTGCGTAAAGGAGTAGA
ATGA

Domains

Predicted by InterproScan.

Competence protein (Competence)

(208-464)

Metallo-beta-lactamase superfamily (Lactamase_B)

(499-672)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q0NB28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Vibrio cholerae strain A1552

78.447

100

0.784
  comEC Vibrio parahaemolyticus RIMD 2210633

41.301

100

0.416
  comEC Vibrio campbellii strain DS40M4

40.16

100

0.404

Multiple sequence alignment