Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   ET951_RS06835 Genome accession   NZ_CP035682
Coordinates   1463727..1464743 (+) Length   338 a.a.
NCBI ID   WP_070381278.1    Uniprot ID   -
Organism   Vibrio mimicus strain 2011V-1073     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1458727..1469743
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET951_RS06820 - 1459277..1460008 (-) 732 WP_148558972.1 glutathione peroxidase -
  ET951_RS06825 oxyR 1460148..1461041 (+) 894 WP_001025915.1 DNA-binding transcriptional regulator OxyR -
  ET951_RS06830 - 1461108..1463585 (-) 2478 WP_192927869.1 penicillin-binding protein 1A -
  ET951_RS06835 pilM 1463727..1464743 (+) 1017 WP_070381278.1 type IV pilus assembly protein PilM Machinery gene
  ET951_RS06840 pilN 1464733..1465317 (+) 585 WP_000903041.1 PilN domain-containing protein Machinery gene
  ET951_RS06845 pilO 1465310..1465900 (+) 591 WP_000135849.1 type 4a pilus biogenesis protein PilO Machinery gene
  ET951_RS06850 pilP 1465890..1466408 (+) 519 WP_154113182.1 pilus assembly protein PilP Machinery gene
  ET951_RS06855 pilQ 1466434..1468149 (+) 1716 WP_187377498.1 type IV pilus secretin PilQ Machinery gene
  ET951_RS06860 aroK 1468336..1468860 (+) 525 WP_000818614.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37559.23 Da        Isoelectric Point: 7.6008

>NTDB_id=343600 ET951_RS06835 WP_070381278.1 1463727..1464743(+) (pilM) [Vibrio mimicus strain 2011V-1073]
MGKTLVTGIDIGHHSIKAVVLKPMGDTYALMGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDNA
VISKVLQIDSDLERREQEFAIYQAFSHQSPFPVEDLSLDFVKVAEKNIARSSTSTFQVYATKKEVVESRLQASKKAGFEP
ILMDVQVHSLLHLWQLASRAYRRPDWMLIDIGHTQSSLCLDFAEKMPFYKDVPLGTRVLGAEPSSTPVLGDSLMQDPTQR
FVNEFVDKVARQIQLFTSVHGAQSLGGLWLSGGGATIAGLEEALHQRLSLPCEVLNPFSLFKTNVSRRKRQVADGQRFST
AAGLALRGLAWLESEHAA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=343600 ET951_RS06835 WP_070381278.1 1463727..1464743(+) (pilM) [Vibrio mimicus strain 2011V-1073]
ATGGGTAAAACATTAGTTACGGGTATTGATATCGGCCACCACAGCATAAAAGCCGTAGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTATGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAAAAAGGGGTTGCCCTTATTTAGTCACAAAGTCGCGATTGCAATTCCGGATAACGCT
GTAATTAGCAAAGTATTACAAATAGATAGCGATCTTGAACGTCGAGAACAGGAATTTGCCATCTATCAGGCTTTCTCTCA
TCAATCTCCATTTCCCGTTGAAGATCTTAGCCTCGATTTCGTCAAAGTGGCGGAAAAAAATATCGCGCGCAGTAGTACAA
GCACCTTCCAAGTCTATGCCACGAAAAAAGAAGTAGTGGAAAGCCGCTTACAAGCGAGTAAGAAAGCGGGGTTTGAGCCG
ATTTTGATGGATGTGCAAGTCCATAGCTTGCTCCATTTATGGCAGCTCGCTAGCCGTGCCTATCGACGACCGGATTGGAT
GCTGATTGATATTGGCCATACCCAGAGTTCATTGTGCCTTGATTTCGCAGAAAAAATGCCTTTTTACAAAGATGTTCCAT
TGGGTACGCGAGTGTTGGGCGCAGAGCCGAGCAGCACTCCCGTATTAGGCGATTCATTAATGCAGGACCCCACTCAGCGT
TTTGTTAACGAATTCGTGGATAAGGTTGCGCGTCAAATTCAGTTGTTTACTTCAGTACATGGGGCACAGAGTCTTGGCGG
TTTATGGCTTTCCGGCGGCGGTGCAACTATTGCTGGACTTGAGGAGGCGCTACACCAACGTTTATCACTTCCCTGTGAAG
TGTTGAATCCATTCTCACTATTCAAAACGAATGTCTCAAGACGCAAGCGTCAAGTGGCTGATGGGCAGCGCTTTAGCACT
GCCGCTGGCCTTGCATTGCGTGGTTTAGCATGGTTGGAGAGTGAACATGCTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

85.799

100

0.858
  pilM Vibrio campbellii strain DS40M4

49.408

100

0.494

Multiple sequence alignment