Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   ET951_RS06845 Genome accession   NZ_CP035682
Coordinates   1465310..1465900 (+) Length   196 a.a.
NCBI ID   WP_000135849.1    Uniprot ID   -
Organism   Vibrio mimicus strain 2011V-1073     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1460310..1470900
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ET951_RS06830 - 1461108..1463585 (-) 2478 WP_192927869.1 penicillin-binding protein 1A -
  ET951_RS06835 pilM 1463727..1464743 (+) 1017 WP_070381278.1 type IV pilus assembly protein PilM Machinery gene
  ET951_RS06840 pilN 1464733..1465317 (+) 585 WP_000903041.1 PilN domain-containing protein Machinery gene
  ET951_RS06845 pilO 1465310..1465900 (+) 591 WP_000135849.1 type 4a pilus biogenesis protein PilO Machinery gene
  ET951_RS06850 pilP 1465890..1466408 (+) 519 WP_154113182.1 pilus assembly protein PilP Machinery gene
  ET951_RS06855 pilQ 1466434..1468149 (+) 1716 WP_187377498.1 type IV pilus secretin PilQ Machinery gene
  ET951_RS06860 aroK 1468336..1468860 (+) 525 WP_000818614.1 shikimate kinase AroK -
  ET951_RS06865 aroB 1468888..1469973 (+) 1086 WP_192927868.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22935.35 Da        Isoelectric Point: 4.4293

>NTDB_id=343602 ET951_RS06845 WP_000135849.1 1465310..1465900(+) (pilO) [Vibrio mimicus strain 2011V-1073]
MASFQELELDEIAEWPLLPQLLVILLLMLLIQGAGYWFYLMPKQDEIVALKQEEETVKATLRIKANKVAALPQIQAQLEE
LKERYDFLLRQLPVQKELASMLASVNQLGLDNSLTFTRIDWGERQTQEFLYRLPLNIELTGNYHDIGDFSEAIAKLPRII
NFDDVDWQRVSQESSTLHFRVKAYTYQFKQEVEDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=343602 ET951_RS06845 WP_000135849.1 1465310..1465900(+) (pilO) [Vibrio mimicus strain 2011V-1073]
ATGGCTAGTTTTCAAGAATTAGAATTGGATGAAATTGCAGAATGGCCACTGCTGCCTCAGCTGCTCGTTATCTTGTTGTT
GATGTTGCTAATTCAAGGCGCTGGCTATTGGTTTTATCTTATGCCCAAGCAAGATGAAATTGTGGCGTTAAAACAAGAAG
AGGAAACGGTAAAAGCGACCTTAAGGATCAAAGCCAATAAAGTAGCAGCGTTACCGCAGATCCAAGCTCAGTTAGAGGAA
TTGAAAGAGCGTTATGATTTTCTATTGCGACAATTACCAGTTCAAAAAGAGCTGGCTAGCATGCTTGCCTCCGTTAACCA
ACTTGGTCTCGATAATTCGCTGACCTTTACGCGTATCGACTGGGGTGAGCGCCAAACACAAGAATTCCTCTACCGTCTAC
CGCTCAATATTGAACTGACGGGTAACTATCACGATATCGGCGATTTTTCAGAGGCGATTGCCAAACTACCTCGCATCATC
AATTTCGATGATGTGGATTGGCAGCGGGTCAGCCAAGAGAGCAGCACTCTACACTTCCGGGTAAAAGCTTATACCTACCA
GTTTAAACAGGAGGTTGAAGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

92.347

100

0.923
  pilO Vibrio campbellii strain DS40M4

70.37

96.429

0.679

Multiple sequence alignment