Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   EWG56_RS14655 Genome accession   NZ_CP035671
Coordinates   2834469..2834966 (+) Length   165 a.a.
NCBI ID   WP_029697681.1    Uniprot ID   A0AAE2ZZ83
Organism   Staphylococcus aureus strain VB31683     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2829469..2839966
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EWG56_RS14615 (EWG56_14615) - 2829514..2830500 (+) 987 WP_000161315.1 ROK family glucokinase -
  EWG56_RS14620 (EWG56_14620) - 2830500..2830829 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EWG56_RS14625 (EWG56_14625) - 2830826..2831449 (+) 624 WP_001223006.1 MBL fold metallo-hydrolase -
  EWG56_RS14630 (EWG56_14630) comGA 2831500..2832474 (+) 975 WP_000697215.1 competence type IV pilus ATPase ComGA Machinery gene
  EWG56_RS14635 (EWG56_14635) comGB 2832446..2833516 (+) 1071 WP_000776403.1 competence type IV pilus assembly protein ComGB Machinery gene
  EWG56_RS14640 (EWG56_14640) comGC 2833530..2833841 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  EWG56_RS14645 (EWG56_14645) comGD 2833819..2834265 (+) 447 WP_075583651.1 competence type IV pilus minor pilin ComGD Machinery gene
  EWG56_RS14650 (EWG56_14650) comGE 2834252..2834551 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  EWG56_RS14655 (EWG56_14655) comGF 2834469..2834966 (+) 498 WP_029697681.1 competence type IV pilus minor pilin ComGF Machinery gene
  EWG56_RS14660 (EWG56_14660) - 2835063..2835209 (+) 147 WP_001789879.1 hypothetical protein -
  EWG56_RS14665 (EWG56_14665) - 2835199..2835723 (+) 525 WP_001015122.1 shikimate kinase -
  EWG56_RS14670 (EWG56_14670) gcvT 2835882..2836973 (+) 1092 WP_000093355.1 glycine cleavage system aminomethyltransferase GcvT -
  EWG56_RS14675 (EWG56_14675) gcvPA 2836993..2838339 (+) 1347 WP_000019688.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  EWG56_RS14680 (EWG56_14680) gcvPB 2838332..2839804 (+) 1473 WP_000202191.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19211.94 Da        Isoelectric Point: 10.2436

>NTDB_id=343502 EWG56_RS14655 WP_029697681.1 2834469..2834966(+) (comGF) [Staphylococcus aureus strain VB31683]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFRGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=343502 EWG56_RS14655 WP_029697681.1 2834469..2834966(+) (comGF) [Staphylococcus aureus strain VB31683]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAGAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(69-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

97.576

100

0.976
  comGF Staphylococcus aureus MW2

97.576

100

0.976

Multiple sequence alignment