Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   EVJ50_RS10405 Genome accession   NZ_CP035632
Coordinates   2122434..2124263 (+) Length   609 a.a.
NCBI ID   WP_191964744.1    Uniprot ID   -
Organism   Synechococcus sp. RSCCF101     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2117434..2129263
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EVJ50_RS10375 (EVJ50_10365) murB 2117917..2118837 (+) 921 WP_150883876.1 UDP-N-acetylmuramate dehydrogenase -
  EVJ50_RS10380 (EVJ50_10370) - 2118908..2119243 (+) 336 WP_150883877.1 YbaB/EbfC family nucleoid-associated protein -
  EVJ50_RS10385 (EVJ50_10375) rsgA 2119224..2120201 (-) 978 WP_150883878.1 ribosome small subunit-dependent GTPase A -
  EVJ50_RS10390 (EVJ50_10380) - 2120158..2120406 (-) 249 WP_225322897.1 sulfurtransferase TusA family protein -
  EVJ50_RS10395 (EVJ50_10385) dnaJ 2120411..2121526 (-) 1116 WP_150883879.1 molecular chaperone DnaJ -
  EVJ50_RS10400 (EVJ50_10390) grpE 2121602..2122336 (-) 735 WP_150883880.1 nucleotide exchange factor GrpE -
  EVJ50_RS10405 (EVJ50_10395) pilB/pilB1 2122434..2124263 (+) 1830 WP_191964744.1 GspE/PulE family protein Machinery gene
  EVJ50_RS10410 (EVJ50_10400) pilT 2124356..2125429 (+) 1074 WP_150883881.1 type IV pilus twitching motility protein PilT Machinery gene
  EVJ50_RS10415 (EVJ50_10405) - 2125453..2126718 (+) 1266 WP_150883882.1 type II secretion system F family protein -
  EVJ50_RS10420 (EVJ50_10410) - 2126720..2127976 (-) 1257 WP_150883883.1 NAD(P)/FAD-dependent oxidoreductase -
  EVJ50_RS10425 (EVJ50_10415) dusA 2128021..2129061 (+) 1041 WP_150883884.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 66644.04 Da        Isoelectric Point: 4.6122

>NTDB_id=343272 EVJ50_RS10405 WP_191964744.1 2122434..2124263(+) (pilB/pilB1) [Synechococcus sp. RSCCF101]
MTLAPARPVPDAIAPEQQRLEVELLLQRRVLQPDELMGWPEAASTEPTLPLQRWRELGGLPVFREGTLLHLAIPTGWGED
EQSALMGAVQELGLEARLHLALSDDIEEAWALAEAQGDAATPTEKHETEAEAPVDLLSEEVLKGFEIQGALEEDSETGDA
PDLEVSLRDTNSSPVITLVDRILMQALSMGASDIHVEPQRKGLQIRFRQDGVLQLQKNPLPPRLIPAVTSRFKIMADLDI
AERRVPQDGRIRRVFKGRPIDFRVNTLPSRFGEKVVLRLLDSSATQLGLDRLVTDQRALGLVRDMGSKPFGIILVTGPTG
SGKSTTLYSLLAERNDPGINISTVEDPIEYTLPGITQTQVNREKGLDFSTALRAFMRQDPDVLLVGETRDLETAKTAIEA
ALTGHLVLTTLHCNDAPSAIARLDEMGVEPFMVSASLLGIISQRLLRRVCPACREPYEPGQEDLGRFGLMASSEAHVTFY
RARKGEGLDGTVCPRCGGSGYKGRVGVYEVLVMNETLASAVSKGAPTETIRRLALEAGMKSLLGYGLDLVREGHTTLEEV
ERMLLTDTGLESERRARALNTQTCEGCGAGLADEWLDCPYCLTPRTMPE

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=343272 EVJ50_RS10405 WP_191964744.1 2122434..2124263(+) (pilB/pilB1) [Synechococcus sp. RSCCF101]
ATGACTCTCGCCCCAGCCAGGCCCGTCCCCGACGCCATCGCTCCCGAGCAGCAGCGGCTTGAGGTGGAACTGCTGCTGCA
GCGGCGGGTGCTGCAACCGGATGAGCTCATGGGCTGGCCGGAGGCGGCGAGCACCGAGCCGACTCTGCCGCTGCAACGCT
GGCGGGAGCTGGGGGGTCTTCCGGTCTTCAGGGAGGGCACCCTGCTCCATCTGGCAATCCCCACCGGATGGGGAGAGGAC
GAGCAGTCCGCGCTGATGGGTGCCGTGCAGGAGCTGGGTCTGGAGGCCAGGCTCCATCTCGCTCTGTCCGATGACATCGA
GGAGGCCTGGGCCTTGGCTGAGGCGCAGGGCGACGCAGCGACACCGACGGAGAAGCACGAGACCGAGGCCGAGGCACCGG
TGGATCTGCTGTCCGAAGAGGTCCTGAAAGGATTTGAGATCCAGGGCGCACTCGAGGAGGACAGCGAGACCGGCGACGCG
CCGGACCTCGAGGTGAGCCTGCGGGACACGAACAGTTCACCCGTGATCACCCTGGTGGATCGGATCCTGATGCAGGCCCT
GAGCATGGGGGCCAGCGACATCCATGTGGAACCGCAGCGCAAGGGGCTCCAGATCCGCTTCAGGCAGGACGGGGTACTCC
AGCTCCAGAAGAATCCGCTGCCCCCGCGGCTGATTCCAGCGGTCACGTCCCGGTTCAAGATCATGGCCGACCTGGACATC
GCGGAGCGCCGGGTCCCCCAGGACGGGCGGATACGGCGGGTGTTCAAGGGACGACCGATTGACTTCCGTGTCAACACCCT
TCCCAGCCGCTTCGGGGAGAAGGTGGTGCTGCGTCTCCTGGACAGCAGTGCCACCCAGCTCGGTCTCGACCGGCTTGTAA
CAGACCAGAGGGCACTCGGTCTGGTGCGGGACATGGGATCCAAACCGTTCGGCATCATTCTCGTGACGGGCCCAACCGGC
TCTGGTAAGTCAACAACTCTGTATTCCCTGCTGGCCGAGCGCAACGATCCGGGCATCAACATCTCCACCGTGGAGGATCC
GATTGAATACACCCTCCCGGGAATCACTCAGACCCAGGTGAACCGTGAAAAGGGTCTTGACTTCTCTACAGCCCTGCGGG
CTTTCATGCGTCAAGATCCCGATGTGCTCCTGGTTGGAGAAACACGCGATCTGGAGACAGCGAAGACGGCCATCGAAGCC
GCCCTCACCGGTCACCTGGTGCTGACCACACTCCACTGCAATGACGCGCCCAGCGCGATCGCTCGTCTCGATGAGATGGG
CGTTGAGCCCTTCATGGTGAGCGCTTCCCTGCTGGGCATCATTTCCCAGCGTCTTCTCCGTCGTGTGTGTCCCGCCTGCA
GAGAGCCCTACGAGCCGGGCCAGGAGGATCTCGGACGTTTCGGGCTGATGGCGTCATCCGAAGCTCATGTGACGTTCTAC
CGGGCGCGCAAGGGCGAAGGCCTTGACGGAACCGTCTGCCCCCGCTGCGGCGGCAGCGGCTATAAGGGCCGCGTTGGGGT
CTACGAGGTCCTGGTGATGAACGAGACCCTCGCCTCGGCCGTGTCGAAGGGAGCTCCGACGGAGACCATCCGGCGACTGG
CTCTCGAAGCCGGCATGAAGAGCCTGCTCGGCTATGGCCTTGATCTGGTCCGGGAAGGGCACACCACGCTGGAGGAGGTG
GAACGGATGCTGCTCACCGACACCGGTCTGGAATCGGAGCGTCGAGCCCGCGCCCTCAACACCCAGACCTGTGAGGGCTG
CGGAGCAGGGCTGGCCGACGAGTGGCTGGACTGCCCGTACTGCCTCACGCCCCGCACCATGCCGGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(182-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

55.148

94.089

0.519

Multiple sequence alignment