Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   F8M43_RS08455 Genome accession   NZ_CP044498
Coordinates   1612909..1614957 (+) Length   682 a.a.
NCBI ID   WP_029317792.1    Uniprot ID   -
Organism   Bacillus subtilis strain ms-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1607909..1619957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F8M43_RS08425 spoVM 1608545..1608625 (+) 81 WP_003221545.1 stage V sporulation protein SpoVM -
  F8M43_RS08430 rpmB 1608698..1608886 (-) 189 WP_003221548.1 50S ribosomal protein L28 -
  F8M43_RS08435 yloU 1609163..1609525 (+) 363 WP_003232054.1 Asp23/Gls24 family envelope stress response protein -
  F8M43_RS08440 fakA 1609541..1611202 (+) 1662 WP_038427652.1 DAK2 domain-containing protein -
  F8M43_RS08445 sdaAB 1611341..1612003 (+) 663 WP_003232050.1 L-serine ammonia-lyase, iron-sulfur-dependent subunit beta -
  F8M43_RS08450 sdaAA 1612029..1612931 (+) 903 WP_003232049.1 L-serine ammonia-lyase, iron-sulfur-dependent, subunit alpha -
  F8M43_RS08455 recG 1612909..1614957 (+) 2049 WP_029317792.1 ATP-dependent DNA helicase RecG Machinery gene
  F8M43_RS08460 fapR 1615066..1615632 (+) 567 WP_003232044.1 transcription factor FapR -
  F8M43_RS08465 plsX 1615646..1616647 (+) 1002 WP_003232041.1 phosphate acyltransferase PlsX -
  F8M43_RS08470 fabD 1616666..1617619 (+) 954 WP_029726491.1 ACP S-malonyltransferase -
  F8M43_RS08475 fabG 1617612..1618352 (+) 741 WP_003232035.1 3-oxoacyl-[acyl-carrier-protein] reductase -
  F8M43_RS08480 acpP 1618436..1618669 (+) 234 WP_003154310.1 acyl carrier protein -
  F8M43_RS08485 rncS 1618809..1619558 (+) 750 WP_003232030.1 ribonuclease III -

Sequence

Protein


Download         Length: 682 a.a.        Molecular weight: 78163.76 Da        Isoelectric Point: 7.4457

>NTDB_id=343203 F8M43_RS08455 WP_029317792.1 1612909..1614957(+) (recG) [Bacillus subtilis strain ms-2]
MKQHQQTSIANIKGIGPETEKTLHELGIYDISDLLNYFPYRYDDYELRDLEEVKHDERVTVEGKVHSEPSLTYYGKKRNR
LTFRLLVGHYLITAVCFNRPYLKKKLSLGSVVTVSGKWDKHRQTISVQELKNGPHQEDKSIEPVYSVKENVTVKMMRRFI
QQALTQYADSLPDPLPEKLRKSYKLPDYYQALKAMHQPETREALKLARRRFVYEEFLLFQLKMQAFRKAEREQTQGIRQR
FSNEELMRFIKSLPFPLTNAQSRVLREITADMSSPYRMNRLLQGDVGSGKTAVAAIALYAAILSGYQGALMVPTEILAEQ
HADSLVSLFEKWDVSVALLTSSVKGKRRKELLERLAAGEIDILVGTHALIQDEVEFKALSLVITDEQHRFGVEQRKKLRN
KGQDPDVLFMTATPIPRTLAITVFGEMDVSVIDEMPAGRKRIETYWVKHDMLDRILAFVEKELKQGRQAYIICPLIEESD
KLDVQNAIDVYNMLSDIFRGKWNVGLMHGKLHSDEKDQVMREFSANHCQILVSTTVVEVGVNVPNATIMVIYDADRFGLS
QLHQLRGRVGRGEHQSFCILMADPKSETGKERMRIMSETNDGFELSEKDLELRGPGDFFGKKQSGMPEFKVADMVHDYRA
LETARQDAANLVASDAFWKEPEYAVLRDELLKSGVMDGEKLS

Nucleotide


Download         Length: 2049 bp        

>NTDB_id=343203 F8M43_RS08455 WP_029317792.1 1612909..1614957(+) (recG) [Bacillus subtilis strain ms-2]
GTGAAACAACATCAGCAAACTAGTATAGCTAACATTAAGGGTATTGGGCCGGAAACAGAAAAAACATTACACGAACTCGG
TATTTATGACATTTCTGATCTTCTGAATTATTTCCCTTATCGCTATGATGACTACGAGCTGAGGGATTTAGAAGAAGTAA
AGCATGATGAAAGAGTCACAGTCGAAGGGAAGGTTCATTCAGAGCCTTCTCTTACCTATTACGGAAAAAAACGAAACAGG
CTGACATTCAGGCTTCTGGTCGGCCACTATTTAATCACAGCCGTATGTTTTAACCGGCCTTATTTGAAGAAGAAGCTTTC
GCTCGGCTCTGTGGTGACGGTTTCAGGTAAATGGGACAAGCACCGCCAAACCATCTCTGTTCAGGAGTTGAAAAACGGGC
CGCATCAAGAAGACAAAAGCATTGAACCAGTGTATTCTGTGAAAGAAAATGTTACCGTCAAAATGATGAGGCGCTTTATT
CAGCAGGCGCTGACCCAATATGCAGACTCACTTCCTGATCCTCTTCCGGAAAAGCTAAGAAAAAGCTATAAACTGCCTGA
CTATTATCAAGCGTTAAAAGCAATGCACCAGCCTGAAACAAGGGAAGCATTAAAGCTTGCCAGACGGCGGTTTGTTTATG
AAGAATTTTTGTTGTTTCAGTTGAAAATGCAGGCGTTCCGAAAGGCGGAAAGAGAGCAGACACAAGGGATACGGCAACGT
TTTTCAAACGAAGAACTCATGAGATTTATCAAAAGCCTCCCGTTTCCCCTCACAAACGCCCAGTCACGCGTTCTTCGCGA
AATAACAGCAGACATGTCTTCTCCATACAGAATGAACCGCCTTCTTCAAGGGGACGTTGGATCAGGAAAAACGGCAGTCG
CCGCCATTGCACTGTATGCCGCGATCCTATCCGGATACCAAGGAGCGCTCATGGTGCCGACAGAAATTCTGGCCGAGCAG
CATGCTGATTCGCTCGTTTCGCTATTTGAAAAATGGGACGTCAGCGTTGCTCTTTTGACAAGCTCTGTTAAAGGGAAGCG
GCGAAAAGAACTGCTTGAGCGTCTTGCGGCGGGTGAGATTGATATTCTTGTAGGAACCCACGCTTTAATCCAAGACGAGG
TGGAGTTTAAGGCGCTGAGTCTCGTTATTACAGATGAACAGCACAGATTTGGAGTTGAGCAGCGCAAAAAGCTTCGGAAC
AAAGGGCAGGATCCCGATGTTCTCTTTATGACAGCCACTCCAATCCCAAGAACGTTAGCGATCACAGTGTTCGGTGAAAT
GGATGTATCTGTCATCGATGAGATGCCGGCAGGACGAAAGAGAATCGAAACCTATTGGGTAAAACATGACATGCTGGATC
GTATATTGGCATTTGTCGAAAAAGAATTAAAGCAAGGCAGGCAGGCTTATATCATCTGTCCGCTGATTGAAGAATCAGAC
AAGCTTGATGTGCAAAACGCCATTGACGTGTACAATATGCTTTCTGATATTTTTCGGGGAAAATGGAATGTCGGCCTTAT
GCATGGAAAGCTGCATTCCGATGAAAAAGACCAGGTCATGAGAGAATTCAGCGCAAATCACTGTCAAATTCTCGTATCAA
CTACTGTTGTGGAGGTTGGCGTGAATGTTCCGAATGCAACGATTATGGTGATTTATGACGCCGACCGTTTCGGGCTATCA
CAGCTTCACCAGCTGCGCGGCCGTGTTGGACGGGGTGAGCATCAATCTTTCTGTATTCTGATGGCTGATCCAAAATCAGA
AACAGGGAAAGAACGGATGAGGATCATGTCGGAGACCAATGACGGTTTCGAGCTGTCTGAAAAGGATCTGGAACTGAGAG
GTCCCGGTGATTTCTTCGGAAAAAAACAAAGCGGAATGCCGGAATTTAAAGTGGCGGACATGGTTCATGATTACAGAGCG
CTTGAAACGGCAAGGCAGGATGCTGCGAATCTTGTGGCTTCTGACGCGTTCTGGAAGGAGCCGGAATACGCTGTGTTAAG
AGATGAATTGCTGAAGAGCGGAGTAATGGACGGGGAAAAATTAAGCTGA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(260-415)

Helicase conserved C-terminal domain (Helicase_C)

(452-572)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(599-670)

RecG wedge domain (RecG_wedge)

(9-165)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Bacillus subtilis subsp. subtilis str. 168

99.853

100

0.999
  recG/mmsA Streptococcus pneumoniae R6

48.968

99.413

0.487
  recG/mmsA Streptococcus pneumoniae R36A

48.968

99.413

0.487
  recG Neisseria meningitidis strain C311

39.542

96.041

0.38