Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   F4V39_RS11430 Genome accession   NZ_CP044148
Coordinates   2166401..2167060 (+) Length   219 a.a.
NCBI ID   WP_001221502.1    Uniprot ID   B7LGG8
Organism   Escherichia coli O157 strain AR-0427     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 2161401..2172060
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F4V39_RS11410 - 2163485..2164456 (-) 972 Protein_2192 ABC transporter substrate-binding protein -
  F4V39_RS11415 - 2164458..2165213 (-) 756 Protein_2193 ABC transporter substrate-binding protein -
  F4V39_RS11420 ygiV 2165322..2165804 (-) 483 WP_000183479.1 GyrI-like domain-containing protein -
  F4V39_RS11425 ygiW 2165857..2166249 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  F4V39_RS11430 ciaR 2166401..2167060 (+) 660 WP_001221502.1 quorum sensing response regulator transcription factor QseB Regulator
  F4V39_RS11435 qseC 2167057..2168406 (+) 1350 WP_000673362.1 quorum sensing histidine kinase QseC -
  F4V39_RS11440 mdaB 2168516..2169097 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  F4V39_RS11445 ygiN 2169128..2169442 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  F4V39_RS11450 - 2169487..2170374 (-) 888 WP_000614950.1 MurR/RpiR family transcriptional regulator -
  F4V39_RS11455 - 2170371..2171318 (-) 948 WP_001301797.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24745.66 Da        Isoelectric Point: 6.6543

>NTDB_id=341141 F4V39_RS11430 WP_001221502.1 2166401..2167060(+) (ciaR) [Escherichia coli O157 strain AR-0427]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=341141 F4V39_RS11430 WP_001221502.1 2166401..2167060(+) (ciaR) [Escherichia coli O157 strain AR-0427]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7LGG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365