Detailed information NTG0340887

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ETL60_RS13100
• Genome accession: NZ_CP035414
• Coordinates: 2431459..2432076 (-)
• Length: 205 a.a.
• NCBI ID: WP_046160608.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM103637
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2426459..2437076

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ETL60_RS13070 (ETL60_13070)	rpsT	2426761..2427027 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ETL60_RS13075 (ETL60_13075)	holA	2427042..2428085 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
ETL60_RS13080 (ETL60_13080)	-	2428125..2428274 (-)	150	WP_003229985.1	hypothetical protein	-
ETL60_RS13085 (ETL60_13085)	yqzM	2428315..2428449 (+)	135	WP_003229983.1	YqzM family protein	-
ETL60_RS13090 (ETL60_13090)	comEC	2428489..2430819 (-)	2331	WP_087614626.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ETL60_RS13095 (ETL60_13095)	comEB	2430823..2431392 (-)	570	WP_003229978.1	ComE operon protein 2	-
ETL60_RS13100 (ETL60_13100)	comEA	2431459..2432076 (-)	618	WP_046160608.1	competence protein ComEA	Machinery gene
ETL60_RS13105 (ETL60_13105)	comER	2432160..2432981 (+)	822	WP_032726195.1	late competence protein ComER	-
ETL60_RS13110 (ETL60_13110)	yqeM	2433047..2433790 (-)	744	WP_046160609.1	class I SAM-dependent methyltransferase	-
ETL60_RS13115 (ETL60_13115)	rsfS	2433787..2434143 (-)	357	WP_014480315.1	ribosome silencing factor	-
ETL60_RS13120 (ETL60_13120)	yqeK	2434161..2434721 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ETL60_RS13125 (ETL60_13125)	nadD	2434711..2435280 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
ETL60_RS13130 (ETL60_13130)	yhbY	2435292..2435582 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ETL60_RS13135 (ETL60_13135)	aroE	2435576..2436418 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21738.48 Da
• Isoelectric Point: 4.7284

>NTDB_id=340887 ETL60_RS13100 WP_046160608.1 2431459..2432076(-) (comEA) [Bacillus subtilis strain SRCM103637]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=340887 ETL60_RS13100 WP_046160608.1 2431459..2432076(-) (comEA) [Bacillus subtilis strain SRCM103637]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376