Detailed information NTG0340729

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ETK71_RS12930
• Genome accession: NZ_CP035411
• Coordinates: 2505357..2505974 (-)
• Length: 205 a.a.
• NCBI ID: WP_041850029.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM103622
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2500357..2510974

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ETK71_RS12900 (ETK71_12900)	rpsT	2500659..2500925 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ETK71_RS12905 (ETK71_12905)	holA	2500940..2501983 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
ETK71_RS12910 (ETK71_12910)	-	2502023..2502172 (-)	150	WP_120363468.1	hypothetical protein	-
ETK71_RS12915 (ETK71_12915)	yqzM	2502213..2502347 (+)	135	WP_003229983.1	YqzM family protein	-
ETK71_RS12920 (ETK71_12920)	comEC	2502387..2504717 (-)	2331	WP_041850028.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ETK71_RS12925 (ETK71_12925)	comEB	2504721..2505290 (-)	570	WP_003229978.1	ComE operon protein 2	-
ETK71_RS12930 (ETK71_12930)	comEA	2505357..2505974 (-)	618	WP_041850029.1	competence protein ComEA	Machinery gene
ETK71_RS12935 (ETK71_12935)	comER	2506058..2506879 (+)	822	WP_041850030.1	late competence protein ComER	-
ETK71_RS12940 (ETK71_12940)	yqeM	2506945..2507688 (-)	744	WP_032726197.1	class I SAM-dependent methyltransferase	-
ETK71_RS12945 (ETK71_12945)	rsfS	2507685..2508041 (-)	357	WP_003237112.1	ribosome silencing factor	-
ETK71_RS12950 (ETK71_12950)	yqeK	2508059..2508619 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ETK71_RS12955 (ETK71_12955)	nadD	2508609..2509178 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
ETK71_RS12960 (ETK71_12960)	yhbY	2509190..2509480 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ETK71_RS12965 (ETK71_12965)	aroE	2509474..2510316 (-)	843	WP_041850031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21821.62 Da
• Isoelectric Point: 4.7220

>NTDB_id=340729 ETK71_RS12930 WP_041850029.1 2505357..2505974(-) (comEA) [Bacillus subtilis strain SRCM103622]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEVQVNLAEILQDGIVVYIPKKGEETAVQQGGGVSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=340729 ETK71_RS12930 WP_041850029.1 2505357..2505974(-) (comEA) [Bacillus subtilis strain SRCM103622]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGTGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGATAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGTGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-117)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	98.049	100	0.98
comEA	Staphylococcus aureus MW2	36.818	100	0.395
comEA	Staphylococcus aureus N315	35.455	100	0.38