Detailed information NTG0340650

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ETK69_RS13400
• Genome accession: NZ_CP035410
• Coordinates: 2682967..2683581 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain SRCM103616
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2677967..2688581

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ETK69_RS13370 (ETK69_13370)	rpsT	2678270..2678536 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ETK69_RS13375 (ETK69_13375)	holA	2678553..2679594 (-)	1042	Protein_2585	DNA polymerase III subunit delta	-
ETK69_RS13380 (ETK69_13380)	-	2679634..2679785 (-)	152	Protein_2586	hypothetical protein	-
ETK69_RS13385 (ETK69_13385)	-	2679826..2679960 (+)	135	WP_003152870.1	YqzM family protein	-
ETK69_RS13390 (ETK69_13390)	comEC	2679979..2682330 (-)	2352	WP_025649830.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ETK69_RS13395 (ETK69_13395)	-	2682331..2682900 (-)	570	WP_003152868.1	ComE operon protein 2	-
ETK69_RS13400 (ETK69_13400)	comEA	2682967..2683581 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
ETK69_RS13405 (ETK69_13405)	comER	2683640..2684461 (+)	822	WP_014305449.1	late competence protein ComER	-
ETK69_RS13410 (ETK69_13410)	-	2684530..2685267 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
ETK69_RS13415 (ETK69_13415)	rsfS	2685264..2685620 (-)	357	WP_003152864.1	ribosome silencing factor	-
ETK69_RS13420 (ETK69_13420)	yqeK	2685617..2686198 (-)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ETK69_RS13425 (ETK69_13425)	-	2686188..2686757 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
ETK69_RS13430 (ETK69_13430)	yhbY	2686768..2687058 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ETK69_RS13435 (ETK69_13435)	aroE	2687052..2687894 (-)	843	WP_017418183.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=340650 ETK69_RS13400 WP_013352942.1 2682967..2683581(-) (comEA) [Bacillus velezensis strain SRCM103616]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=340650 ETK69_RS13400 WP_013352942.1 2682967..2683581(-) (comEA) [Bacillus velezensis strain SRCM103616]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363