Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ETK61_RS14100 Genome accession   NZ_CP035406
Coordinates   2587445..2588062 (-) Length   205 a.a.
NCBI ID   WP_004398514.1    Uniprot ID   A0AAE2SLR2
Organism   Bacillus subtilis strain SRCM103612     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2582445..2593062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETK61_RS14070 (ETK61_14075) rpsT 2582763..2583029 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  ETK61_RS14075 (ETK61_14080) holA 2583044..2584087 (-) 1044 WP_014480310.1 DNA polymerase III subunit delta -
  ETK61_RS14080 (ETK61_14085) - 2584127..2584276 (-) 150 WP_003229985.1 hypothetical protein -
  ETK61_RS14085 (ETK61_14090) yqzM 2584317..2584451 (+) 135 WP_003229983.1 YqzM family protein -
  ETK61_RS14090 (ETK61_14095) comEC 2584491..2586805 (-) 2315 Protein_2719 DNA internalization-related competence protein ComEC/Rec2 -
  ETK61_RS14095 (ETK61_14100) comEB 2586809..2587378 (-) 570 WP_003229978.1 ComE operon protein 2 -
  ETK61_RS14100 (ETK61_14105) comEA 2587445..2588062 (-) 618 WP_004398514.1 competence protein ComEA Machinery gene
  ETK61_RS14105 (ETK61_14110) comER 2588146..2588967 (+) 822 WP_014480313.1 late competence protein ComER -
  ETK61_RS14110 (ETK61_14115) yqeM 2589033..2589776 (-) 744 WP_029317885.1 class I SAM-dependent methyltransferase -
  ETK61_RS14115 (ETK61_14120) rsfS 2589773..2590129 (-) 357 WP_014480315.1 ribosome silencing factor -
  ETK61_RS14120 (ETK61_14125) yqeK 2590147..2590707 (-) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ETK61_RS14125 (ETK61_14130) nadD 2590697..2591266 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  ETK61_RS14130 (ETK61_14135) yhbY 2591278..2591568 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  ETK61_RS14135 (ETK61_14140) aroE 2591562..2592404 (-) 843 WP_038429328.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21769.45 Da        Isoelectric Point: 4.7220

>NTDB_id=340572 ETK61_RS14100 WP_004398514.1 2587445..2588062(-) (comEA) [Bacillus subtilis strain SRCM103612]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=340572 ETK61_RS14100 WP_004398514.1 2587445..2588062(-) (comEA) [Bacillus subtilis strain SRCM103612]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

100

100

1
  comEA Staphylococcus aureus MW2

37.273

100

0.4
  comEA Staphylococcus aureus N315

36.364

100

0.39

Multiple sequence alignment