Detailed information NTG0340269

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ES969_RS13420
• Genome accession: NZ_CP035402
• Coordinates: 2463555..2464172 (-)
• Length: 205 a.a.
• NCBI ID: WP_046160608.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM103576
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2458555..2469172

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ES969_RS13390 (ES969_13390)	rpsT	2458857..2459123 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ES969_RS13395 (ES969_13395)	holA	2459138..2460181 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
ES969_RS13400 (ES969_13400)	-	2460221..2460370 (-)	150	WP_003229985.1	hypothetical protein	-
ES969_RS13405 (ES969_13405)	yqzM	2460411..2460545 (+)	135	WP_003229983.1	YqzM family protein	-
ES969_RS13410 (ES969_13410)	comEC	2460585..2462915 (-)	2331	WP_069837640.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ES969_RS13415 (ES969_13415)	comEB	2462919..2463488 (-)	570	WP_003229978.1	ComE operon protein 2	-
ES969_RS13420 (ES969_13420)	comEA	2463555..2464172 (-)	618	WP_046160608.1	competence protein ComEA	Machinery gene
ES969_RS13425 (ES969_13425)	comER	2464256..2465077 (+)	822	WP_032726195.1	late competence protein ComER	-
ES969_RS13430 (ES969_13430)	yqeM	2465143..2465886 (-)	744	WP_046160609.1	class I SAM-dependent methyltransferase	-
ES969_RS13435 (ES969_13435)	rsfS	2465883..2466239 (-)	357	WP_014480315.1	ribosome silencing factor	-
ES969_RS13440 (ES969_13440)	yqeK	2466257..2466817 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ES969_RS13445 (ES969_13445)	nadD	2466807..2467376 (-)	570	WP_041333756.1	nicotinate-nucleotide adenylyltransferase	-
ES969_RS13450 (ES969_13450)	yhbY	2467388..2467678 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ES969_RS13455 (ES969_13455)	aroE	2467672..2468514 (-)	843	WP_129134001.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21738.48 Da
• Isoelectric Point: 4.7284

>NTDB_id=340269 ES969_RS13420 WP_046160608.1 2463555..2464172(-) (comEA) [Bacillus subtilis strain SRCM103576]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKLFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=340269 ES969_RS13420 WP_046160608.1 2463555..2464172(-) (comEA) [Bacillus subtilis strain SRCM103576]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGAGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTTTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTTATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376