Detailed information NTG0340108

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ETA18_RS12950
• Genome accession: NZ_CP035400
• Coordinates: 2503819..2504436 (-)
• Length: 205 a.a.
• NCBI ID: WP_032722140.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM103835
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2498819..2509436

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ETA18_RS12920 (ETA18_12920)	rpsT	2499121..2499387 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ETA18_RS12925 (ETA18_12925)	holA	2499402..2500445 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
ETA18_RS12930 (ETA18_12930)	-	2500485..2500634 (-)	150	WP_003229985.1	hypothetical protein	-
ETA18_RS12935 (ETA18_12935)	yqzM	2500675..2500809 (+)	135	WP_003229983.1	YqzM family protein	-
ETA18_RS12940 (ETA18_12940)	comEC	2500849..2503179 (-)	2331	WP_128993168.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ETA18_RS12945 (ETA18_12945)	comEB	2503183..2503752 (-)	570	WP_003229978.1	ComE operon protein 2	-
ETA18_RS12950 (ETA18_12950)	comEA	2503819..2504436 (-)	618	WP_032722140.1	competence protein ComEA	Machinery gene
ETA18_RS12955 (ETA18_12955)	comER	2504520..2505341 (+)	822	WP_014480313.1	late competence protein ComER	-
ETA18_RS12960 (ETA18_12960)	yqeM	2505407..2506150 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
ETA18_RS12965 (ETA18_12965)	rsfS	2506147..2506503 (-)	357	WP_041333759.1	ribosome silencing factor	-
ETA18_RS12970 (ETA18_12970)	yqeK	2506521..2507081 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ETA18_RS12975 (ETA18_12975)	nadD	2507071..2507640 (-)	570	WP_041333756.1	nicotinate-nucleotide adenylyltransferase	-
ETA18_RS12980 (ETA18_12980)	yhbY	2507652..2507942 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ETA18_RS12985 (ETA18_12985)	aroE	2507936..2508778 (-)	843	WP_029317883.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21748.46 Da
• Isoelectric Point: 4.8354

>NTDB_id=340108 ETA18_RS12950 WP_032722140.1 2503819..2504436(-) (comEA) [Bacillus subtilis strain SRCM103835]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVRHPGVYELRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=340108 ETA18_RS12950 WP_032722140.1 2503819..2504436(-) (comEA) [Bacillus subtilis strain SRCM103835]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAATGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAATTGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	98.537	100	0.985
comEA	Staphylococcus aureus MW2	36.364	100	0.39
comEA	Staphylococcus aureus N315	35.455	100	0.38