Detailed information NTG0340031

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ETA12_RS13030
• Genome accession: NZ_CP035399
• Coordinates: 2629144..2629758 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain SRCM103788
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2624144..2634758

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ETA12_RS13000 (ETA12_13000)	rpsT	2624447..2624713 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ETA12_RS13005 (ETA12_13005)	holA	2624730..2625771 (-)	1042	Protein_2508	DNA polymerase III subunit delta	-
ETA12_RS13010 (ETA12_13010)	-	2625811..2625962 (-)	152	Protein_2509	hypothetical protein	-
ETA12_RS13015 (ETA12_13015)	-	2626003..2626137 (+)	135	WP_003152870.1	YqzM family protein	-
ETA12_RS13020 (ETA12_13020)	comEC	2626156..2628507 (-)	2352	WP_025649830.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ETA12_RS13025 (ETA12_13025)	-	2628508..2629077 (-)	570	WP_003152868.1	ComE operon protein 2	-
ETA12_RS13030 (ETA12_13030)	comEA	2629144..2629758 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
ETA12_RS13035 (ETA12_13035)	comER	2629817..2630638 (+)	822	WP_014305449.1	late competence protein ComER	-
ETA12_RS13040 (ETA12_13040)	-	2630707..2631444 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
ETA12_RS13045 (ETA12_13045)	rsfS	2631441..2631797 (-)	357	WP_003152864.1	ribosome silencing factor	-
ETA12_RS13050 (ETA12_13050)	yqeK	2631794..2632375 (-)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ETA12_RS13055 (ETA12_13055)	-	2632365..2632934 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
ETA12_RS13060 (ETA12_13060)	yhbY	2632945..2633235 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ETA12_RS13065 (ETA12_13065)	aroE	2633229..2634071 (-)	843	WP_017418183.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=340031 ETA12_RS13030 WP_013352942.1 2629144..2629758(-) (comEA) [Bacillus velezensis strain SRCM103788]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=340031 ETA12_RS13030 WP_013352942.1 2629144..2629758(-) (comEA) [Bacillus velezensis strain SRCM103788]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363