Detailed information NTG0339713

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ES966_RS13025
• Genome accession: NZ_CP035393
• Coordinates: 2628928..2629542 (-)
• Length: 204 a.a.
• NCBI ID: WP_013352942.1
• Uniprot ID: A0A411A874
• Organism: Bacillus velezensis strain SRCM103691
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2623928..2634542

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ES966_RS12995 (ES966_12995)	rpsT	2624231..2624497 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ES966_RS13000 (ES966_13000)	holA	2624514..2625555 (-)	1042	Protein_2508	DNA polymerase III subunit delta	-
ES966_RS13005 (ES966_13005)	-	2625595..2625746 (-)	152	Protein_2509	hypothetical protein	-
ES966_RS13010 (ES966_13010)	-	2625787..2625921 (+)	135	WP_003152870.1	YqzM family protein	-
ES966_RS13015 (ES966_13015)	comEC	2625940..2628291 (-)	2352	WP_025649830.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ES966_RS13020 (ES966_13020)	-	2628292..2628861 (-)	570	WP_003152868.1	ComE operon protein 2	-
ES966_RS13025 (ES966_13025)	comEA	2628928..2629542 (-)	615	WP_013352942.1	helix-hairpin-helix domain-containing protein	Machinery gene
ES966_RS13030 (ES966_13030)	comER	2629601..2630422 (+)	822	WP_014305449.1	late competence protein ComER	-
ES966_RS13035 (ES966_13035)	-	2630491..2631228 (-)	738	WP_014305450.1	class I SAM-dependent methyltransferase	-
ES966_RS13040 (ES966_13040)	rsfS	2631225..2631581 (-)	357	WP_003152864.1	ribosome silencing factor	-
ES966_RS13045 (ES966_13045)	yqeK	2631578..2632159 (-)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ES966_RS13050 (ES966_13050)	-	2632149..2632717 (-)	569	Protein_2518	nicotinate-nucleotide adenylyltransferase	-
ES966_RS13055 (ES966_13055)	yhbY	2632728..2633018 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
ES966_RS13060 (ES966_13060)	aroE	2633012..2633854 (-)	843	WP_017418183.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22190.27 Da
• Isoelectric Point: 5.4841

>NTDB_id=339713 ES966_RS13025 WP_013352942.1 2628928..2629542(-) (comEA) [Bacillus velezensis strain SRCM103691]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=339713 ES966_RS13025 WP_013352942.1 2628928..2629542(-) (comEA) [Bacillus velezensis strain SRCM103691]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: A0A411A874

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363