Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ES965_RS18325 Genome accession   NZ_CP035391
Coordinates   3505385..3506776 (-) Length   463 a.a.
NCBI ID   WP_124059234.1    Uniprot ID   -
Organism   Bacillus subtilis strain SRCM103689     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3500385..3511776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES965_RS18290 (ES965_18290) flgL 3500556..3501452 (-) 897 WP_015714797.1 flagellar hook-associated protein FlgL -
  ES965_RS18295 (ES965_18295) flgK 3501463..3502986 (-) 1524 WP_124059232.1 flagellar hook-associated protein FlgK -
  ES965_RS18300 (ES965_18300) flgN 3503005..3503487 (-) 483 WP_077671017.1 flagellar protein FlgN -
  ES965_RS18305 (ES965_18305) flgM 3503503..3503769 (-) 267 WP_024572695.1 flagellar biosynthesis anti-sigma factor FlgM -
  ES965_RS18310 (ES965_18310) yvyF 3503850..3504269 (-) 420 WP_024572694.1 TIGR03826 family flagellar region protein -
  ES965_RS18315 (ES965_18315) comFC 3504343..3505065 (-) 723 WP_024572693.1 comF operon protein ComFC Machinery gene
  ES965_RS18320 (ES965_18320) comFB 3505029..3505325 (-) 297 WP_015483764.1 late competence protein ComFB -
  ES965_RS18325 (ES965_18325) comFA 3505385..3506776 (-) 1392 WP_124059234.1 ATP-dependent helicase ComFA Machinery gene
  ES965_RS18330 (ES965_18330) fakBA 3506882..3507727 (-) 846 WP_003227986.1 DegV family protein -
  ES965_RS18335 (ES965_18335) degU 3507825..3508514 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  ES965_RS18340 (ES965_18340) degS 3508597..3509754 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  ES965_RS18345 (ES965_18345) yvyE 3509971..3510624 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52446.51 Da        Isoelectric Point: 10.0155

>NTDB_id=339652 ES965_RS18325 WP_124059234.1 3505385..3506776(-) (comFA) [Bacillus subtilis strain SRCM103689]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWGGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=339652 ES965_RS18325 WP_124059234.1 3505385..3506776(-) (comFA) [Bacillus subtilis strain SRCM103689]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGGCGGCAAGCTTTCAAGCGGACAACAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTATGGAGGAAGTGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATTTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATTTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(326-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

99.136

100

0.991

Multiple sequence alignment