Detailed information NTG0339634

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ES965_RS12870
• Genome accession: NZ_CP035391
• Coordinates: 2480743..2481360 (-)
• Length: 205 a.a.
• NCBI ID: WP_129092543.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM103689
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2475743..2486360

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ES965_RS12840 (ES965_12840)	rpsT	2476045..2476311 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
ES965_RS12845 (ES965_12845)	holA	2476326..2477369 (-)	1044	WP_129092541.1	DNA polymerase III subunit delta	-
ES965_RS12850 (ES965_12850)	-	2477409..2477558 (-)	150	WP_003229985.1	hypothetical protein	-
ES965_RS12855 (ES965_12855)	yqzM	2477599..2477733 (+)	135	WP_003229983.1	YqzM family protein	-
ES965_RS12860 (ES965_12860)	comEC	2477773..2480103 (-)	2331	WP_129092542.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ES965_RS12865 (ES965_12865)	comEB	2480107..2480676 (-)	570	WP_003229978.1	ComE operon protein 2	-
ES965_RS12870 (ES965_12870)	comEA	2480743..2481360 (-)	618	WP_129092543.1	competence protein ComEA	Machinery gene
ES965_RS12875 (ES965_12875)	comER	2481444..2482265 (+)	822	WP_004398597.1	late competence protein ComER	-
ES965_RS12880 (ES965_12880)	yqeM	2482331..2483074 (-)	744	WP_070547553.1	class I SAM-dependent methyltransferase	-
ES965_RS12885 (ES965_12885)	rsfS	2483071..2483427 (-)	357	WP_014477391.1	ribosome silencing factor	-
ES965_RS12890 (ES965_12890)	yqeK	2483445..2484005 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ES965_RS12895 (ES965_12895)	nadD	2483995..2484564 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
ES965_RS12900 (ES965_12900)	yhbY	2484576..2484866 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
ES965_RS12905 (ES965_12905)	aroE	2484860..2485702 (-)	843	WP_129092544.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21753.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=339634 ES965_RS12870 WP_129092543.1 2480743..2481360(-) (comEA) [Bacillus subtilis strain SRCM103689]
MNWLNQHKKAIILAASAAVFTTIMIFLATGKNKEPVKQAAPTEAENTVVKQEANNDESNEIIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=339634 ES965_RS12870 WP_129092543.1 2480743..2481360(-) (comEA) [Bacillus subtilis strain SRCM103689]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAACGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGCACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAATAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGTGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	98.049	100	0.98
comEA	Staphylococcus aureus MW2	37.557	100	0.405
comEA	Staphylococcus aureus N315	36.486	100	0.395