Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ES965_RS12425 Genome accession   NZ_CP035391
Coordinates   2400262..2401332 (-) Length   356 a.a.
NCBI ID   WP_024573393.1    Uniprot ID   A0A165AJM7
Organism   Bacillus subtilis strain SRCM103689     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2395262..2406332
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ES965_RS12380 (ES965_12380) tapA 2395740..2396501 (-) 762 WP_129092525.1 amyloid fiber anchoring/assembly protein TapA -
  ES965_RS12385 (ES965_12385) yqzG 2396774..2397100 (+) 327 WP_024573388.1 YqzG/YhdC family protein -
  ES965_RS12390 (ES965_12390) spoIITA 2397142..2397321 (-) 180 WP_014480252.1 YqzE family protein -
  ES965_RS12395 (ES965_12395) comGG 2397392..2397766 (-) 375 WP_032726157.1 ComG operon protein ComGG Machinery gene
  ES965_RS12400 (ES965_12400) comGF 2397767..2398150 (-) 384 WP_129092526.1 ComG operon protein ComGF Machinery gene
  ES965_RS12405 (ES965_12405) comGE 2398176..2398523 (-) 348 WP_123373078.1 ComG operon protein 5 Machinery gene
  ES965_RS12410 (ES965_12410) comGD 2398507..2398938 (-) 432 WP_024573390.1 comG operon protein ComGD Machinery gene
  ES965_RS12415 (ES965_12415) comGC 2398928..2399224 (-) 297 WP_129092527.1 comG operon protein ComGC Machinery gene
  ES965_RS12420 (ES965_12420) comGB 2399238..2400275 (-) 1038 WP_129092528.1 comG operon protein ComGB Machinery gene
  ES965_RS12425 (ES965_12425) comGA 2400262..2401332 (-) 1071 WP_024573393.1 competence protein ComGA Machinery gene
  ES965_RS12430 (ES965_12430) - 2401545..2401742 (-) 198 WP_124058597.1 hypothetical protein -
  ES965_RS12435 (ES965_12435) corA 2401744..2402697 (-) 954 WP_032677123.1 magnesium transporter CorA -
  ES965_RS12440 (ES965_12440) yqhB 2402840..2404168 (+) 1329 WP_003230150.1 hemolysin family protein -
  ES965_RS12445 (ES965_12445) rsbRD 2404221..2405057 (-) 837 WP_015384093.1 RsbT co-antagonist protein RsbRD -
  ES965_RS12455 (ES965_12455) mgsR 2405280..2405660 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  ES965_RS12460 (ES965_12460) yqgY 2405892..2406137 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40472.67 Da        Isoelectric Point: 9.0175

>NTDB_id=339630 ES965_RS12425 WP_024573393.1 2400262..2401332(-) (comGA) [Bacillus subtilis strain SRCM103689]
MDSIEKLSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=339630 ES965_RS12425 WP_024573393.1 2400262..2401332(-) (comGA) [Bacillus subtilis strain SRCM103689]
TTGGATTCAATAGAAAAGTTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGAAAGACTACCACATTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGATCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGATCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTGACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACCAGGAGAGCTAGTGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A165AJM7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.719

100

0.997
  pilB Glaesserella parasuis strain SC1401

43.878

82.584

0.362

Multiple sequence alignment