Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   F3P14_RS03500 Genome accession   NZ_CP043942
Coordinates   719591..720250 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain AR216.2b     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 714591..725250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F3P14_RS03475 (F3P14_03475) parE 714682..716574 (+) 1893 WP_000195292.1 DNA topoisomerase IV subunit B -
  F3P14_RS03480 (F3P14_03480) ygiN 716622..716936 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  F3P14_RS03485 (F3P14_03485) mdaB 716967..717548 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  F3P14_RS03490 (F3P14_03490) ygiZ 717867..718199 (+) 333 WP_000917685.1 DUF2645 family protein -
  F3P14_RS03495 (F3P14_03495) qseC 718245..719594 (-) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  F3P14_RS03500 (F3P14_03500) ciaR 719591..720250 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  F3P14_RS03505 (F3P14_03505) ygiW 720402..720794 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  F3P14_RS03510 (F3P14_03510) ygiV 720847..721329 (+) 483 WP_000183505.1 GyrI-like domain-containing protein -
  F3P14_RS03515 (F3P14_03515) mqsR 721534..721830 (+) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  F3P14_RS03520 (F3P14_03520) mqsA 721832..722227 (+) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  F3P14_RS03525 (F3P14_03525) ygiS 722360..723967 (+) 1608 WP_087523553.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=339451 F3P14_RS03500 WP_001221493.1 719591..720250(-) (ciaR) [Escherichia coli strain AR216.2b]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=339451 F3P14_RS03500 WP_001221493.1 719591..720250(-) (ciaR) [Escherichia coli strain AR216.2b]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment