Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   FS841_RS15970 Genome accession   NZ_CP043937
Coordinates   3347393..3348052 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain RM14516     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3342393..3353052
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FS841_RS15950 (FS841_16090) plsC 3342539..3343276 (-) 738 WP_000965711.1 1-acylglycerol-3-phosphate O-acyltransferase -
  FS841_RS15955 (FS841_16095) parC 3343510..3345768 (-) 2259 WP_001281890.1 DNA topoisomerase IV subunit A -
  FS841_RS15960 (FS841_16100) ygiV 3346314..3346796 (-) 483 WP_000183492.1 AraC family transcriptional regulator -
  FS841_RS15965 (FS841_16105) ygiW 3346849..3347241 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  FS841_RS15970 (FS841_16110) ciaR 3347393..3348052 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  FS841_RS15975 (FS841_16115) qseC 3348049..3349398 (+) 1350 WP_001544946.1 quorum sensing histidine kinase QseC -
  FS841_RS15980 (FS841_16120) mdaB 3349508..3350089 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  FS841_RS15985 (FS841_16125) ygiN 3350120..3350434 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  FS841_RS15990 (FS841_16130) - 3350479..3351366 (-) 888 WP_000614956.1 MurR/RpiR family transcriptional regulator -
  FS841_RS15995 (FS841_16135) - 3351363..3352310 (-) 948 WP_001377068.1 iron-siderophore ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=339401 FS841_RS15970 WP_001221493.1 3347393..3348052(+) (ciaR) [Escherichia coli strain RM14516]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=339401 FS841_RS15970 WP_001221493.1 3347393..3348052(+) (ciaR) [Escherichia coli strain RM14516]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTCGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGTGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365