Detailed information NTG0338867

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: F0T11_RS10245
• Genome accession: NZ_CP043816
• Coordinates: 1969677..1970903 (+)
• Length: 408 a.a.
• NCBI ID: WP_003697786.1
• Uniprot ID: B4RPS5
• Organism: Neisseria gonorrhoeae strain NG251
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1964677..1975903

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
F0T11_RS10220 (F0T11_11415)	dksA	1965853..1966269 (-)	417	WP_003692108.1	RNA polymerase-binding protein DksA	-
F0T11_RS10225 (F0T11_11420)	proC	1966417..1967208 (-)	792	WP_003696395.1	pyrroline-5-carboxylate reductase	-
F0T11_RS10230 (F0T11_11425)	-	1967257..1967664 (-)	408	WP_003690202.1	hypothetical protein	-
F0T11_RS10235 (F0T11_11430)	-	1967664..1968350 (-)	687	WP_003690201.1	YggS family pyridoxal phosphate-dependent enzyme	-
F0T11_RS10240 (F0T11_11435)	pilT	1968470..1969513 (+)	1044	WP_003690200.1	type IV pilus twitching motility protein PilT	Machinery gene
F0T11_RS10245 (F0T11_11440)	pilU	1969677..1970903 (+)	1227	WP_003697786.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
F0T11_RS10250 (F0T11_11445)	yccS	1971020..1973170 (+)	2151	WP_003690198.1	YccS family putative transporter	-

Sequence

Protein

• Length: 408 a.a.
• Molecular weight: 45668.30 Da
• Isoelectric Point: 7.2909

>NTDB_id=338867 F0T11_RS10245 WP_003697786.1 1969677..1970903(+) (pilU) [Neisseria gonorrhoeae strain NG251]
MNTDNLHDILDETVQVYSQKKQSRSETPAEIGAHFHPLLDRLCETAEAQNASDILISKGFPPSLKINSALTPQPQKALTG
EETAAIAASTMNAEQSEIFRRDGEINYSVQSRSGTRYRANAYYSQGSAGLVLRRINHVIPQMRELGLPEKLKDLAVAPRG
LLIIVGPTGSGKSTTMATMLEHRNKTLPGHIVTIEDPIEFIYKPRRCIFTQREIGVDTINWQTAVQNAMRQSPDVVCIGE
VRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERILNFYPKEQHNQILIDIALNLTGIICQRLALKKDKTGRTAVVDLL
INTPAIQDFILKGDLMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGKTPELLYDR
VNGLNLIS

Nucleotide

• Length: 1227 bp

>NTDB_id=338867 F0T11_RS10245 WP_003697786.1 1969677..1970903(+) (pilU) [Neisseria gonorrhoeae strain NG251]
ATGAATACCGATAACCTGCACGACATCTTGGACGAAACGGTTCAAGTGTATTCTCAAAAAAAACAAAGCCGATCCGAAAC
CCCGGCCGAAATCGGCGCACACTTCCACCCGCTGCTCGACCGCCTGTGCGAAACCGCAGAAGCACAAAACGCGTCCGACA
TCCTTATCAGCAAAGGATTCCCGCCCTCGTTGAAAATCAACAGCGCATTAACCCCGCAGCCGCAAAAGGCTCTGACGGGC
GAGGAAACCGCCGCCATCGCCGCATCGACGATGAACGCCGAACAATCGGAAATATTCCGGCGCGACGGCGAAATCAACTA
CTCCGTCCAATCGCGCAGCGGCACGCGCTACCGCGCCAACGCCTACTACAGCCAAGGCAGCGCAGGTTTGGTTTTGCGGC
GCATCAACCACGTCATCCCGCAAATGCGGGAATTGGGCTTGCCCGAAAAACTCAAAGACCTCGCCGTCGCACCGCGCGGG
CTGCTGATTATCGTCGGGCCTACCGGTTCGGGCAAATCCACCACGATGGCGACTATGCTCGAACACCGCAACAAAACCCT
GCCCGGCCATATCGTTACCATCGAAGACCCGATTGAGTTTATCTACAAACCGCGCCGCTGCATCTTTACCCAGCGCGAAA
TCGGCGTCGACACCATAAACTGGCAGACGGCGGTACAAAACGCTATGCGCCAATCCCCAGACGTGGTCTGTATCGGCGAA
GTCCGCAGCAGGGAAAGTATGGAATACGCGATGCAGCTCGCCCAAACCGGCCACCTGTGCATTTTTACGCTCCACGCCAA
CACCGCGCCGCAGTCGCTCGAACGCATACTCAACTTCTACCCCAAAGAACAGCACAACCAAATACTGATTGACATCGCCC
TCAATCTGACCGGCATCATCTGCCAACGCCTCGCCCTCAAAAAAGACAAAACGGGCAGGACGGCGGTTGTCGACTTGCTC
ATCAACACGCCCGCCATCCAAGACTTCATCCTGAAGGGCGACCTGATGAACATCAGTAAAATCATGGAAACCGCCAAAAC
CGACGGAATGCAGACGATGGACCAAAACCTTTTCGAACTGTACCGTCACGGCATCATCAGTTACGAAGAAGCCCTGCGCC
AGTCCGTTTCCGCCAACAACCTGCGATTGCACATCCAACTGCACAAAGAAGGCAAAACGCCCGAACTCCTTTACGACAGG
GTCAACGGTCTCAACCTCATTTCCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (49-305)

3D structure

• AlphaFold DB: B4RPS5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	44.875	88.48	0.397
pilU	Pseudomonas stutzeri DSM 10701	45.225	87.255	0.395
pilU	Vibrio cholerae strain A1552	47.024	82.353	0.387