Detailed information NTG0338306

Bioinformatically predicted

Overview

• Name: dprA/cilB/dalA
• Type: Machinery gene
• Locus tag: EQH20_RS05540
• Genome accession: NZ_CP035260
• Coordinates: 1130385..1131233 (-)
• Length: 282 a.a.
• NCBI ID: WP_000705304.1
• Uniprot ID: A0A0E7P1X9
• Organism: Streptococcus pneumoniae strain TVO_1901925
• Function: ssDNA binding; loading RecA onto ssDNA; competence shut-off (predicted from homology) DNA processing Competence regulation

Genomic Context

Location: 1125385..1136233

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH20_RS05525 (EQH20_05825)	-	1125624..1125983 (-)	360	WP_001220357.1	YbaN family protein	-
EQH20_RS05530 (EQH20_05830)	topA	1126204..1128291 (-)	2088	WP_000179648.1	type I DNA topoisomerase	-
EQH20_RS05535 (EQH20_05835)	-	1128458..1129501 (-)	1044	WP_001812710.1	TIGR00341 family protein	-
EQH20_RS05540 (EQH20_05840)	dprA/cilB/dalA	1130385..1131233 (-)	849	WP_000705304.1	DNA-processing protein DprA	Machinery gene
EQH20_RS05545 (EQH20_05845)	-	1131319..1132008 (-)	690	WP_061647411.1	sugar phosphate nucleotidyltransferase	-
EQH20_RS05550 (EQH20_05850)	-	1132020..1132898 (-)	879	WP_000837618.1	DMT family transporter	-
EQH20_RS05555 (EQH20_05855)	licA	1132888..1133757 (-)	870	WP_000411215.1	choline kinase LicA	-
EQH20_RS05560 (EQH20_05860)	-	1133774..1134796 (-)	1023	WP_000609885.1	ribitol-5-phosphate dehydrogenase	-
EQH20_RS05565 (EQH20_05865)	-	1134801..1135508 (-)	708	WP_000638502.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 31076.75 Da
• Isoelectric Point: 5.4047

>NTDB_id=338306 EQH20_RS05540 WP_000705304.1 1130385..1131233(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain TVO_1901925]
MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADISGCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDC
YPWDLSEIYDAPVLLFYKGNLDLLKFPKVAVVGSRACSKQGAKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRGVIVAEAKMRSGSLITCERAMEE
GRDVFAIPGSILDGLSDGCHHLIQEGAKLLTSGQDVLAEFEF

Nucleotide

• Length: 849 bp

>NTDB_id=338306 EQH20_RS05540 WP_000705304.1 1130385..1131233(-) (dprA/cilB/dalA) [Streptococcus pneumoniae strain TVO_1901925]
ATGAAAATCACAAACTATGAAATCTATAAGTTAAAAAAATCAGGTTTGACCAATCAACAGATTTTGAAAGTGCTAGAATA
CGGTGAAAATGTTGATCAGGAGCTTTTGTTGGGTGATATTGCAGATATCTCAGGTTGCCGTAATCCAGCCGTTTTTATGG
AACGTTATTTTCAGATAGACGATGCGCATTTGTCGAAAGAGTTTCAAAAATTTCCATCTTTCTCTATTTTAGATGACTGT
TATCCTTGGGATTTGAGTGAAATATATGATGCGCCTGTACTTTTATTTTACAAGGGAAATCTTGACCTCCTGAAATTCCC
GAAGGTAGCGGTCGTGGGCAGTCGTGCTTGTAGCAAACAGGGAGCTAAGTCAGTTGAAAAAGTCATTCAAGGCTTGGAAA
ATGAACTGGTTATTGTCAGTGGTCTGGCCAAGGGCATTGACACAGCAGCTCATATGGCAGCTCTTCAGAATGGCGGAAAA
ACCATTGCAGTGATTGGAACAGGACTGGATGTGTTTTATCCTAAAGCCAATAAACGCTTGCAAGACTACATCGGCAATGA
CCATCTGGTTCTAAGTGAATATGGACCTGGCGAACAACCTCTGAAATTTCATTTTCCTGCCCGTAATCGCATCATTGCTG
GACTTTGTCGTGGTGTGATTGTAGCAGAGGCTAAGATGCGTTCAGGTAGTCTCATTACGTGTGAGCGAGCAATGGAAGAA
GGACGCGATGTCTTTGCTATTCCTGGTAGCATTTTAGATGGACTATCAGACGGTTGCCATCATTTGATTCAAGAAGGAGC
AAAATTGCTCACCAGTGGGCAAGATGTTCTTGCGGAATTTGAATTTTAA

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (75-271)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (3-64)

3D structure

• AlphaFold DB: A0A0E7P1X9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae D39	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae R6	99.645	100	0.996
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	99.645	100	0.996
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	98.227	100	0.982
dprA/cilB/dalA	Streptococcus mitis SK321	97.518	100	0.975
dprA	Streptococcus mutans UA159	63.214	99.291	0.628
dprA	Lactococcus lactis subsp. cremoris KW2	55.357	99.291	0.55
dprA	Staphylococcus aureus N315	37.456	100	0.376