Detailed information NTG0336469

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EQZ01_RS13275
• Genome accession: NZ_CP035231
• Coordinates: 2538197..2538814 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain SRCM103571
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2533197..2543814

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQZ01_RS13245 (EQZ01_13245)	rpsT	2533499..2533765 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
EQZ01_RS13250 (EQZ01_13250)	holA	2533780..2534823 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
EQZ01_RS13255 (EQZ01_13255)	-	2534863..2535012 (-)	150	WP_003229985.1	hypothetical protein	-
EQZ01_RS13260 (EQZ01_13260)	yqzM	2535053..2535187 (+)	135	WP_003229983.1	YqzM family protein	-
EQZ01_RS13265 (EQZ01_13265)	comEC	2535227..2537557 (-)	2331	WP_128738024.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQZ01_RS13270 (EQZ01_13270)	comEB	2537561..2538130 (-)	570	WP_003229978.1	ComE operon protein 2	-
EQZ01_RS13275 (EQZ01_13275)	comEA	2538197..2538814 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
EQZ01_RS13280 (EQZ01_13280)	comER	2538898..2539719 (+)	822	WP_014480313.1	late competence protein ComER	-
EQZ01_RS13285 (EQZ01_13285)	yqeM	2539785..2540528 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
EQZ01_RS13290 (EQZ01_13290)	rsfS	2540525..2540881 (-)	357	WP_041333759.1	ribosome silencing factor	-
EQZ01_RS13295 (EQZ01_13295)	yqeK	2540899..2541459 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EQZ01_RS13300 (EQZ01_13300)	nadD	2541449..2542018 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
EQZ01_RS13305 (EQZ01_13305)	yhbY	2542030..2542320 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
EQZ01_RS13310 (EQZ01_13310)	aroE	2542314..2543156 (-)	843	WP_128738025.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=336469 EQZ01_RS13275 WP_004398514.1 2538197..2538814(-) (comEA) [Bacillus subtilis strain SRCM103571]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=336469 EQZ01_RS13275 WP_004398514.1 2538197..2538814(-) (comEA) [Bacillus subtilis strain SRCM103571]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39