Detailed information NTG0336390

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EQY76_RS13080
• Genome accession: NZ_CP035230
• Coordinates: 2455860..2456477 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain SRCM103551
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2450860..2461477

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQY76_RS13050 (EQY76_13050)	rpsT	2451162..2451428 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
EQY76_RS13055 (EQY76_13055)	holA	2451443..2452486 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
EQY76_RS13060 (EQY76_13060)	-	2452526..2452675 (-)	150	WP_003229985.1	hypothetical protein	-
EQY76_RS13065 (EQY76_13065)	yqzM	2452716..2452850 (+)	135	WP_003229983.1	YqzM family protein	-
EQY76_RS13070 (EQY76_13070)	comEC	2452890..2455220 (-)	2331	WP_046381195.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQY76_RS13075 (EQY76_13075)	comEB	2455224..2455793 (-)	570	WP_046381196.1	ComE operon protein 2	-
EQY76_RS13080 (EQY76_13080)	comEA	2455860..2456477 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
EQY76_RS13085 (EQY76_13085)	comER	2456561..2457382 (+)	822	WP_014480313.1	late competence protein ComER	-
EQY76_RS13090 (EQY76_13090)	yqeM	2457448..2458191 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
EQY76_RS13095 (EQY76_13095)	rsfS	2458188..2458544 (-)	357	WP_014480315.1	ribosome silencing factor	-
EQY76_RS13100 (EQY76_13100)	yqeK	2458562..2459122 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EQY76_RS13105 (EQY76_13105)	nadD	2459112..2459681 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
EQY76_RS13110 (EQY76_13110)	yhbY	2459693..2459983 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
EQY76_RS13115 (EQY76_13115)	aroE	2459977..2460819 (-)	843	WP_041351695.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=336390 EQY76_RS13080 WP_004398514.1 2455860..2456477(-) (comEA) [Bacillus subtilis strain SRCM103551]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=336390 EQY76_RS13080 WP_004398514.1 2455860..2456477(-) (comEA) [Bacillus subtilis strain SRCM103551]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39