Detailed information NTG0336231

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EQW70_RS14075
• Genome accession: NZ_CP035226
• Coordinates: 2660085..2660702 (-)
• Length: 205 a.a.
• NCBI ID: WP_103804084.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM103517
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2655085..2665702

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQW70_RS14045 (EQW70_14045)	rpsT	2655385..2655651 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
EQW70_RS14050 (EQW70_14050)	holA	2655666..2656709 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
EQW70_RS14055 (EQW70_14055)	-	2656749..2656862 (-)	114	WP_122060479.1	hypothetical protein	-
EQW70_RS14060 (EQW70_14060)	yqzM	2656939..2657073 (+)	135	WP_003229983.1	YqzM family protein	-
EQW70_RS14065 (EQW70_14065)	comEC	2657114..2659444 (-)	2331	WP_128740584.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQW70_RS14070 (EQW70_14070)	comEB	2659448..2660017 (-)	570	WP_003229978.1	ComE operon protein 2	-
EQW70_RS14075 (EQW70_14075)	comEA	2660085..2660702 (-)	618	WP_103804084.1	competence protein ComEA	Machinery gene
EQW70_RS14080 (EQW70_14080)	comER	2660786..2661607 (+)	822	WP_032726195.1	late competence protein ComER	-
EQW70_RS14085 (EQW70_14085)	yqeM	2661673..2662416 (-)	744	WP_032726197.1	class I SAM-dependent methyltransferase	-
EQW70_RS14090 (EQW70_14090)	rsfS	2662413..2662769 (-)	357	WP_014480315.1	ribosome silencing factor	-
EQW70_RS14095 (EQW70_14095)	yqeK	2662787..2663347 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EQW70_RS14100 (EQW70_14100)	nadD	2663337..2663906 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
EQW70_RS14105 (EQW70_14105)	yhbY	2663918..2664208 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
EQW70_RS14110 (EQW70_14110)	aroE	2664202..2665044 (-)	843	WP_014480317.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21737.54 Da
• Isoelectric Point: 4.9680

>NTDB_id=336231 EQW70_RS14075 WP_103804084.1 2660085..2660702(-) (comEA) [Bacillus subtilis strain SRCM103517]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSVGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=336231 EQW70_RS14075 WP_103804084.1 2660085..2660702(-) (comEA) [Bacillus subtilis strain SRCM103517]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGTTGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.073	100	0.971
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376