Detailed information NTG0335452

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EQJ08_RS12710
• Genome accession: NZ_CP035167
• Coordinates: 2478361..2478978 (-)
• Length: 205 a.a.
• NCBI ID: WP_032726193.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM104008
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2473361..2483978

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQJ08_RS12680 (EQJ08_12680)	rpsT	2473662..2473928 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
EQJ08_RS12685 (EQJ08_12685)	holA	2473943..2474986 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
EQJ08_RS12690 (EQJ08_12690)	-	2475026..2475139 (-)	114	WP_122060479.1	hypothetical protein	-
EQJ08_RS12695 (EQJ08_12695)	yqzM	2475216..2475350 (+)	135	WP_003229983.1	YqzM family protein	-
EQJ08_RS12700 (EQJ08_12700)	comEC	2475391..2477721 (-)	2331	WP_128441738.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQJ08_RS12705 (EQJ08_12705)	comEB	2477725..2478294 (-)	570	WP_003229978.1	ComE operon protein 2	-
EQJ08_RS12710 (EQJ08_12710)	comEA	2478361..2478978 (-)	618	WP_032726193.1	competence protein ComEA	Machinery gene
EQJ08_RS12715 (EQJ08_12715)	comER	2479062..2479883 (+)	822	WP_128441739.1	late competence protein ComER	-
EQJ08_RS12720 (EQJ08_12720)	yqeM	2479949..2480692 (-)	744	WP_032726197.1	class I SAM-dependent methyltransferase	-
EQJ08_RS12725 (EQJ08_12725)	rsfS	2480689..2481045 (-)	357	WP_003237112.1	ribosome silencing factor	-
EQJ08_RS12730 (EQJ08_12730)	yqeK	2481063..2481623 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EQJ08_RS12735 (EQJ08_12735)	nadD	2481613..2482182 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
EQJ08_RS12740 (EQJ08_12740)	yhbY	2482194..2482484 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
EQJ08_RS12745 (EQJ08_12745)	aroE	2482478..2483320 (-)	843	WP_041850031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21753.50 Da
• Isoelectric Point: 4.8417

>NTDB_id=335452 EQJ08_RS12710 WP_032726193.1 2478361..2478978(-) (comEA) [Bacillus subtilis strain SRCM104008]
MNWLNQHKKAIVLAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESSETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTKEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEEITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=335452 EQJ08_RS12710 WP_032726193.1 2478361..2478978(-) (comEA) [Bacillus subtilis strain SRCM104008]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTGTTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAGCGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCAGGCGTTTATGAAATGCGTACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCAGGCGGGACCAAAGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGAAATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-118)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	97.561	100	0.976
comEA	Staphylococcus aureus MW2	35.909	100	0.385
comEA	Staphylococcus aureus N315	35	100	0.376