Detailed information NTG0335374

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EQI87_RS13205
• Genome accession: NZ_CP035166
• Coordinates: 2526832..2527449 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain SRCM103971
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2521832..2532449

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQI87_RS13175 (EQI87_13175)	rpsT	2522134..2522400 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
EQI87_RS13180 (EQI87_13180)	holA	2522415..2523458 (-)	1044	WP_014480310.1	DNA polymerase III subunit delta	-
EQI87_RS13185 (EQI87_13185)	-	2523498..2523647 (-)	150	WP_003229985.1	hypothetical protein	-
EQI87_RS13190 (EQI87_13190)	yqzM	2523688..2523822 (+)	135	WP_003229983.1	YqzM family protein	-
EQI87_RS13195 (EQI87_13195)	comEC	2523862..2526192 (-)	2331	WP_046381195.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQI87_RS13200 (EQI87_13200)	comEB	2526196..2526765 (-)	570	WP_046381196.1	ComE operon protein 2	-
EQI87_RS13205 (EQI87_13205)	comEA	2526832..2527449 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
EQI87_RS13210 (EQI87_13210)	comER	2527533..2528354 (+)	822	WP_014480313.1	late competence protein ComER	-
EQI87_RS13215 (EQI87_13215)	yqeM	2528420..2529163 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
EQI87_RS13220 (EQI87_13220)	rsfS	2529160..2529516 (-)	357	WP_014480315.1	ribosome silencing factor	-
EQI87_RS13225 (EQI87_13225)	yqeK	2529534..2530094 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EQI87_RS13230 (EQI87_13230)	nadD	2530084..2530653 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
EQI87_RS13235 (EQI87_13235)	yhbY	2530665..2530955 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
EQI87_RS13240 (EQI87_13240)	aroE	2530949..2531791 (-)	843	WP_041351695.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=335374 EQI87_RS13205 WP_004398514.1 2526832..2527449(-) (comEA) [Bacillus subtilis strain SRCM103971]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=335374 EQI87_RS13205 WP_004398514.1 2526832..2527449(-) (comEA) [Bacillus subtilis strain SRCM103971]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39