Detailed information NTG0335290

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EQI56_RS13530
• Genome accession: NZ_CP035165
• Coordinates: 2591158..2591775 (-)
• Length: 205 a.a.
• NCBI ID: WP_041850029.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain SRCM103881
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2586158..2596775

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQI56_RS13500 (EQI56_13500)	rpsT	2586460..2586726 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
EQI56_RS13505 (EQI56_13505)	holA	2586741..2587784 (-)	1044	WP_029317888.1	DNA polymerase III subunit delta	-
EQI56_RS13510 (EQI56_13510)	-	2587824..2587973 (-)	150	WP_120363468.1	hypothetical protein	-
EQI56_RS13515 (EQI56_13515)	yqzM	2588014..2588148 (+)	135	WP_003229983.1	YqzM family protein	-
EQI56_RS13520 (EQI56_13520)	comEC	2588188..2590518 (-)	2331	WP_041850028.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQI56_RS13525 (EQI56_13525)	comEB	2590522..2591091 (-)	570	WP_003229978.1	ComE operon protein 2	-
EQI56_RS13530 (EQI56_13530)	comEA	2591158..2591775 (-)	618	WP_041850029.1	competence protein ComEA	Machinery gene
EQI56_RS13535 (EQI56_13535)	comER	2591859..2592680 (+)	822	WP_041850030.1	late competence protein ComER	-
EQI56_RS13540 (EQI56_13540)	yqeM	2592746..2593489 (-)	744	WP_032726197.1	class I SAM-dependent methyltransferase	-
EQI56_RS13545 (EQI56_13545)	rsfS	2593486..2593842 (-)	357	WP_003237112.1	ribosome silencing factor	-
EQI56_RS13550 (EQI56_13550)	yqeK	2593860..2594420 (-)	561	WP_014480316.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EQI56_RS13555 (EQI56_13555)	nadD	2594410..2594979 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
EQI56_RS13560 (EQI56_13560)	yhbY	2594991..2595281 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
EQI56_RS13565 (EQI56_13565)	aroE	2595275..2596117 (-)	843	WP_041850031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21821.62 Da
• Isoelectric Point: 4.7220

>NTDB_id=335290 EQI56_RS13530 WP_041850029.1 2591158..2591775(-) (comEA) [Bacillus subtilis strain SRCM103881]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEVQVNLAEILQDGIVVYIPKKGEETAVQQGGGVSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=335290 EQI56_RS13530 WP_041850029.1 2591158..2591775(-) (comEA) [Bacillus subtilis strain SRCM103881]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGTGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGATAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGTGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (63-117)

• Helix-hairpin-helix motif (HHH_3) (142-203)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	98.049	100	0.98
comEA	Staphylococcus aureus MW2	36.818	100	0.395
comEA	Staphylococcus aureus N315	35.455	100	0.38