Detailed information NTG0334972

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EQH88_RS13245
• Genome accession: NZ_CP035161
• Coordinates: 2539604..2540221 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis strain SRCM103862
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2534604..2545221

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EQH88_RS13215 (EQH88_13215)	rpsT	2534913..2535179 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
EQH88_RS13220 (EQH88_13220)	holA	2535194..2536237 (-)	1044	WP_015714286.1	DNA polymerase III subunit delta	-
EQH88_RS13225 (EQH88_13225)	-	2536277..2536426 (-)	150	WP_003229985.1	hypothetical protein	-
EQH88_RS13230 (EQH88_13230)	yqzM	2536467..2536601 (+)	135	WP_003229983.1	YqzM family protein	-
EQH88_RS13235 (EQH88_13235)	comEC	2536641..2538971 (-)	2331	WP_080477815.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EQH88_RS13240 (EQH88_13240)	comEB	2538968..2539537 (-)	570	WP_015714288.1	ComE operon protein 2	-
EQH88_RS13245 (EQH88_13245)	comEA	2539604..2540221 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
EQH88_RS13250 (EQH88_13250)	comER	2540305..2541126 (+)	822	WP_004398597.1	late competence protein ComER	-
EQH88_RS13255 (EQH88_13255)	yqeM	2541192..2541935 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
EQH88_RS13260 (EQH88_13260)	rsfS	2541932..2542288 (-)	357	WP_003229971.1	ribosome silencing factor	-
EQH88_RS13265 (EQH88_13265)	yqeK	2542306..2542866 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EQH88_RS13270 (EQH88_13270)	nadD	2542856..2543425 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
EQH88_RS13275 (EQH88_13275)	yhbY	2543437..2543727 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
EQH88_RS13280 (EQH88_13280)	aroE	2543721..2544563 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=334972 EQH88_RS13245 WP_004398514.1 2539604..2540221(-) (comEA) [Bacillus subtilis strain SRCM103862]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=334972 EQH88_RS13245 WP_004398514.1 2539604..2540221(-) (comEA) [Bacillus subtilis strain SRCM103862]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39