Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilY1   Type   Machinery gene
Locus tag   EPY81_RS02400 Genome accession   NZ_CP035109
Coordinates   478742..482596 (-) Length   1284 a.a.
NCBI ID   WP_002116849.1    Uniprot ID   A0A8I1HEG1
Organism   Acinetobacter pittii strain NQ-003     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 473742..487596
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EPY81_RS02365 (EPY81_02375) lip 474481..475422 (+) 942 WP_014207808.1 lipase family alpha/beta hydrolase -
  EPY81_RS02370 (EPY81_02380) rplS 475474..475842 (-) 369 WP_002116654.1 50S ribosomal protein L19 -
  EPY81_RS02375 (EPY81_02385) trmD 476062..476817 (-) 756 WP_002116964.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -
  EPY81_RS02380 (EPY81_02390) rimM 476860..477408 (-) 549 WP_002116736.1 ribosome maturation factor RimM -
  EPY81_RS02385 (EPY81_02395) rpsP 477428..477679 (-) 252 WP_000260334.1 30S ribosomal protein S16 -
  EPY81_RS02390 (EPY81_02400) pilE 477827..478252 (-) 426 WP_002117055.1 type IV pilin protein Machinery gene
  EPY81_RS02395 (EPY81_02405) pilY2 478249..478731 (-) 483 WP_002116770.1 type IV pilin protein Machinery gene
  EPY81_RS02400 (EPY81_02410) pilY1 478742..482596 (-) 3855 WP_002116849.1 PilC/PilY family type IV pilus protein Machinery gene
  EPY81_RS02405 (EPY81_02415) pilX 482608..483417 (-) 810 WP_002117088.1 PilX N-terminal domain-containing pilus assembly protein Machinery gene
  EPY81_RS02410 (EPY81_02420) pilW 483414..484415 (-) 1002 WP_002116811.1 PilW family protein Machinery gene
  EPY81_RS02415 (EPY81_02425) pilV 484415..484975 (-) 561 WP_080564113.1 type IV pilus modification protein PilV Machinery gene
  EPY81_RS02420 (EPY81_02430) fimU 484969..485442 (-) 474 WP_016802756.1 pilus assembly FimT family protein Machinery gene
  EPY81_RS02425 (EPY81_02435) ispH 485612..486562 (-) 951 WP_002116992.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase -
  EPY81_RS02430 (EPY81_02440) gmk 486685..487314 (+) 630 WP_002116973.1 guanylate kinase -

Sequence

Protein


Download         Length: 1284 a.a.        Molecular weight: 137703.23 Da        Isoelectric Point: 7.5995

>NTDB_id=334537 EPY81_RS02400 WP_002116849.1 478742..482596(-) (pilY1) [Acinetobacter pittii strain NQ-003]
MKLKLKNFKPNNLWYAICSSSVAFTWLMTSSVVQASDLQIYASPTAGKKTIVMMLDTSGSMTNNSYGENRLAMLKNGMNA
FLASNNPVLNDTRVGLGNFSANGDSRSGQVLVAAAPLGDASTLNTVGSQRYKLKQAVANLTAGGSTPSAHAYAEAAAYLM
GTTTYSEANYAIRKDSYIKRVRKSDNRTEYSYCTNYRDSQIDTTNLWQPCRSNNYWSNWSTSNPGVDAATAYDTSSDWTY
SYTYYYTTFNYAVANADSGIPKSKSNDTTSNPNIVVDRNASNLNAIYQSPLPAVTNRQSCDGQGIYFLSDGEPNNTTNTR
SATVMSTALGSTLGANFTCSGGLSNTTADSGWACMGEFAKRLFDKTKNPAGVSIQTAFVGFGSDFSSLSSSDVKNACRLS
SRTQSDRKGDDACSPNQTTNAVAAPGYGNGGFFPTQSAQGVTDSVIAFINNLEKVPLQPLTTGAISVPYDALNPKNLQEY
GYLRALEPNPANTYLTWRGNLKKYHVVLSGGNAGAFEANTGGLVYNASGAFRTGTKDYWNSSNYNDGGNVFLGGSYAKVP
LPIAGQNETRDEDGNIIKYYYAVQTKIRNLFTDVSAVAADGSLTKISTSGTNLLKIPAAPPEGTNPFDSVANTASYVLGK
FNASTGQDILKAFPIGLKLKILNYLGYSTDISATTLPSSLVTSNEPYLSMGGSIHSLPVQLTYNGTLDENGNLTSAREQS
ILYGTMEGGLHLVDASTGVEQMAFVPADILNDPIASKALVVGQSDASAPAHGMDGAWVSDPAYSITTTGSGSSAVSKVTA
KQMNIYGGMRMGGSSYYGLNVLNPVSPKLLFRVGADQTDYSRMGQSWSKPVLANIRYNGVIKRVMIVGGGYDQCYENPNI
TLSNSCFTNGKAKGNAVYIIDAKTGERLWWTSDTGSNIDNSNMKHSIVSRISTLDRDADGLVDHLYFGDLGGQIFRIDLN
NNQTKTNSTYSSFGIRVVRLANLATNDSTYDASNDYTAGNAPRFYEPPTVTIHDYGIRTFITVGIASGDRSTPLDVYPLT
GREGMSPSTALSGRPVNNVYGIIDKDFIKKNLMSLADNQLETKDITRLTLRKNPQILRSGETRVAQIFFPNTGVGQSGWY
RSLSSMSDGTEKANNSFRIKGGLKAFEEPMAVTGTLIVPVYDPQGTGIVAADPCLPRVVGETDQQTFCLPFGACLNSDGS
IDQNKENGSGFKTQTGTNCPVGASECNKNVIGSGIRGVTFVPTEDNPPATNSCGKLKLSGNESGTGEWQCTSHLVPARWY
ERYR

Nucleotide


Download         Length: 3855 bp        

>NTDB_id=334537 EPY81_RS02400 WP_002116849.1 478742..482596(-) (pilY1) [Acinetobacter pittii strain NQ-003]
GTGAAACTAAAATTGAAGAATTTTAAGCCAAATAATTTATGGTATGCCATCTGTTCGAGTTCAGTTGCTTTTACATGGCT
AATGACAAGCTCCGTTGTGCAGGCCAGTGATTTACAAATTTATGCTTCACCTACAGCTGGTAAAAAAACCATTGTGATGA
TGTTGGATACATCAGGAAGTATGACCAACAATAGTTATGGCGAAAACCGTCTGGCTATGCTTAAAAATGGTATGAATGCT
TTTTTAGCAAGTAATAATCCTGTTTTAAATGACACTCGTGTGGGGTTGGGAAATTTCTCTGCAAATGGTGATAGCCGAAG
TGGGCAGGTTCTAGTGGCTGCTGCTCCTTTAGGAGATGCGAGTACTTTAAATACAGTGGGTTCTCAACGTTATAAATTAA
AACAGGCTGTTGCAAATTTAACTGCTGGCGGGTCAACGCCTTCAGCACATGCTTATGCGGAAGCTGCTGCTTATTTGATG
GGCACAACGACCTATTCAGAGGCAAATTATGCCATTCGAAAAGATAGCTATATAAAGCGTGTGCGAAAATCTGATAATAG
AACGGAATACTCTTATTGTACGAATTATCGCGATTCACAAATCGATACTACAAATCTATGGCAACCTTGTCGCTCTAATA
ACTATTGGAGTAACTGGTCAACTTCTAATCCGGGTGTGGATGCGGCAACTGCTTATGATACTTCGTCGGATTGGACCTAT
TCTTATACTTATTACTACACCACCTTTAATTATGCTGTAGCAAATGCAGATAGTGGTATCCCAAAATCAAAATCAAACGA
TACAACCAGCAATCCCAATATTGTTGTAGATAGAAATGCCTCTAATTTAAATGCTATTTACCAATCTCCTTTACCTGCGG
TGACCAATCGCCAGAGTTGTGATGGGCAAGGAATCTACTTTTTATCAGATGGGGAGCCGAACAATACAACAAATACGCGT
TCTGCAACTGTCATGTCGACAGCCCTAGGCAGCACTTTGGGAGCAAATTTTACTTGTTCTGGAGGTTTGTCTAACACTAC
AGCAGACTCTGGTTGGGCCTGTATGGGGGAATTTGCAAAAAGATTATTTGATAAAACAAAAAACCCAGCTGGGGTTTCTA
TTCAAACAGCATTTGTCGGTTTTGGTAGTGACTTTTCTAGTTTAAGTTCTTCTGATGTAAAAAATGCCTGTCGTTTAAGT
TCGAGAACCCAATCTGACCGAAAAGGAGATGATGCGTGTTCACCGAATCAGACCACAAATGCGGTCGCTGCACCTGGTTA
TGGGAATGGAGGTTTTTTCCCTACCCAAAGTGCGCAAGGTGTAACAGACAGTGTTATCGCATTTATTAATAACTTAGAAA
AAGTTCCGTTACAACCCTTAACCACTGGCGCTATCTCAGTTCCTTATGATGCACTAAACCCTAAAAATTTACAGGAATAT
GGTTACTTACGGGCTTTAGAGCCAAACCCAGCTAACACCTACTTAACATGGCGTGGAAATTTAAAGAAATATCATGTTGT
TTTATCAGGGGGCAATGCAGGTGCTTTTGAAGCTAACACTGGTGGGTTGGTTTACAATGCAAGTGGGGCTTTTAGAACGG
GTACGAAAGATTATTGGAATAGCTCTAATTATAATGATGGAGGTAACGTCTTTTTAGGTGGTTCATATGCAAAAGTTCCT
TTACCAATTGCTGGACAAAATGAAACACGTGATGAAGATGGAAATATCATAAAATATTATTACGCAGTACAAACTAAAAT
CCGCAACTTATTTACTGATGTTTCCGCTGTTGCGGCAGATGGTAGCTTAACGAAAATATCGACTTCTGGAACTAATTTGC
TAAAAATCCCAGCTGCTCCCCCAGAAGGAACTAATCCTTTTGATAGTGTGGCTAATACAGCAAGTTATGTTTTAGGAAAA
TTTAATGCATCAACTGGACAAGATATTTTAAAAGCTTTCCCGATTGGCTTGAAATTAAAAATATTAAATTATCTAGGTTA
CTCAACTGATATTAGCGCTACGACTTTACCTTCGTCATTAGTTACATCGAATGAACCCTATTTATCGATGGGCGGTAGTA
TTCATTCTTTACCGGTGCAGCTTACCTATAATGGAACATTAGATGAAAATGGGAATTTAACATCTGCGCGTGAACAATCT
ATTCTCTATGGAACGATGGAAGGTGGTCTGCATCTTGTGGATGCTTCAACTGGCGTAGAACAAATGGCTTTCGTTCCGGC
TGATATATTAAATGATCCAATTGCATCAAAAGCTTTAGTGGTTGGCCAAAGTGATGCCTCAGCTCCTGCTCACGGTATGG
ATGGGGCTTGGGTATCTGACCCTGCATATAGTATTACTACAACGGGAAGCGGTAGCTCAGCAGTATCAAAAGTAACTGCA
AAGCAAATGAATATTTATGGTGGCATGCGAATGGGTGGCAGCAGCTATTATGGCTTAAATGTCTTAAATCCTGTCTCACC
TAAGCTCCTGTTTAGAGTAGGAGCAGATCAAACTGATTATAGTCGCATGGGGCAGAGTTGGTCTAAACCTGTACTTGCAA
ATATACGTTATAACGGAGTTATTAAACGGGTTATGATTGTTGGTGGTGGCTACGACCAATGTTATGAAAACCCGAATATT
ACCTTGAGTAATTCTTGCTTTACGAATGGAAAAGCAAAAGGGAATGCTGTTTATATTATTGATGCTAAAACTGGGGAACG
CTTATGGTGGACTAGTGATACAGGCTCTAATATAGATAACTCTAATATGAAGCACAGTATTGTTAGTCGCATTAGTACCT
TAGATCGAGATGCTGATGGTTTGGTAGACCATTTATATTTTGGCGATTTAGGCGGGCAAATTTTCCGTATCGATCTTAAT
AATAACCAGACAAAAACAAATTCAACTTATAGCAGTTTCGGTATTCGAGTTGTTCGATTAGCGAATTTAGCAACAAATGA
CTCAACTTATGATGCTTCGAATGACTATACCGCTGGGAATGCGCCGCGTTTTTATGAACCGCCAACAGTGACAATTCATG
ATTATGGAATCCGCACTTTTATTACTGTCGGTATTGCTTCAGGTGACCGTAGTACACCTTTAGATGTTTATCCGCTTACA
GGTCGTGAGGGGATGTCACCGAGCACCGCATTAAGCGGAAGACCTGTAAACAATGTCTATGGGATTATCGATAAAGACTT
TATCAAAAAGAATTTAATGTCTTTAGCTGATAACCAACTTGAAACAAAAGATATTACTCGATTGACGCTTCGAAAAAATC
CACAAATTTTACGGTCTGGGGAAACACGAGTAGCACAGATTTTCTTTCCAAACACTGGGGTGGGGCAAAGTGGTTGGTAT
CGCTCACTTTCTAGTATGAGCGATGGCACAGAGAAAGCTAATAATAGTTTCCGTATTAAAGGTGGACTGAAAGCTTTTGA
AGAACCTATGGCCGTTACGGGGACTTTAATTGTGCCTGTTTATGATCCGCAGGGAACAGGCATTGTAGCGGCAGACCCAT
GTTTACCACGTGTAGTAGGTGAAACTGACCAGCAGACGTTCTGTTTACCATTTGGCGCCTGCCTTAATTCTGATGGGTCA
ATTGATCAAAATAAAGAGAATGGAAGTGGATTCAAAACACAAACTGGTACTAATTGCCCAGTAGGAGCTTCCGAATGTAA
TAAAAACGTTATTGGCTCCGGTATTCGTGGCGTAACATTTGTACCAACGGAGGATAACCCACCTGCGACTAATAGTTGTG
GGAAATTAAAGCTGTCTGGAAACGAGAGTGGAACGGGCGAGTGGCAATGTACAAGTCACTTAGTTCCTGCGCGCTGGTAT
GAGCGTTATCGCTAA

Domains

Predicted by InterproScan.

Neisseria PilC beta-propeller domain (T4P_PilY1)

(714-965)

von Willebrand factor type A domain (VWA_2)

(51-161)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilY1 Acinetobacter baumannii D1279779

84.884

100

0.853

Multiple sequence alignment