Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   FYA20_RS08640 Genome accession   NZ_CP043181
Coordinates   1800005..1800664 (+) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli O2:H6 strain PG20180057     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 1795005..1805664
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FYA20_RS08625 (FYA20_08625) ygiS 1797210..1798817 (-) 1608 WP_148885897.1 ABC transporter substrate-binding protein -
  FYA20_RS08630 (FYA20_08630) ygiV 1798926..1799408 (-) 483 WP_000183508.1 GyrI-like domain-containing protein -
  FYA20_RS08635 (FYA20_08635) ygiW 1799461..1799853 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  FYA20_RS08640 (FYA20_08640) ciaR 1800005..1800664 (+) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  FYA20_RS08645 (FYA20_08645) qseC 1800661..1802010 (+) 1350 WP_000673367.1 quorum sensing histidine kinase QseC -
  FYA20_RS08650 (FYA20_08650) ygiZ 1802056..1802388 (-) 333 WP_000914696.1 DUF2645 family protein -
  FYA20_RS25520 - 1802395..1803105 (-) 711 WP_000834024.1 hypothetical protein -
  FYA20_RS25525 - 1803108..1803587 (-) 480 WP_000065329.1 Hcp family type VI secretion system effector -
  FYA20_RS08660 (FYA20_08660) mdaB 1803837..1804418 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  FYA20_RS08665 (FYA20_08665) ygiN 1804449..1804763 (+) 315 WP_000633738.1 putative quinol monooxygenase -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=334411 FYA20_RS08640 WP_001221493.1 1800005..1800664(+) (ciaR) [Escherichia coli O2:H6 strain PG20180057]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=334411 FYA20_RS08640 WP_001221493.1 1800005..1800664(+) (ciaR) [Escherichia coli O2:H6 strain PG20180057]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAAGCGCTATATAGCGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCGGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTATTGATCCTGACTGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGCAACGTCATGC
TAGACCCTGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACGCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365