Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEC   Type   Machinery gene
Locus tag   EO946_RS12930 Genome accession   NZ_CP034943
Coordinates   2510968..2513298 (-) Length   776 a.a.
NCBI ID   WP_003226149.1    Uniprot ID   -
Organism   Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2505968..2518298
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO946_RS12895 (EO946_12895) - 2506306..2506644 (-) 339 WP_003226161.1 YqxA family protein -
  EO946_RS12900 (EO946_12900) spoIIP 2506661..2507866 (-) 1206 WP_003226159.1 spore autolysin SpoIIP -
  EO946_RS12905 (EO946_12905) gpr 2507929..2509035 (-) 1107 WP_003226157.1 GPR endopeptidase -
  EO946_RS12910 (EO946_12910) rpsT 2509239..2509505 (+) 267 WP_003226155.1 30S ribosomal protein S20 -
  EO946_RS12915 (EO946_12915) holA 2509520..2510563 (-) 1044 WP_003226154.1 DNA polymerase III subunit delta -
  EO946_RS12920 (EO946_12920) - 2510603..2510752 (-) 150 WP_136975859.1 hypothetical protein -
  EO946_RS12925 (EO946_12925) - 2510793..2510927 (+) 135 WP_003226152.1 YqzM family protein -
  EO946_RS12930 (EO946_12930) comEC 2510968..2513298 (-) 2331 WP_003226149.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  EO946_RS12935 (EO946_12935) - 2513302..2513871 (-) 570 WP_003226147.1 ComE operon protein 2 -
  EO946_RS12940 (EO946_12940) comEA 2513938..2514555 (-) 618 WP_003226145.1 helix-hairpin-helix domain-containing protein Machinery gene
  EO946_RS12945 (EO946_12945) comER 2514639..2515460 (+) 822 WP_003226143.1 late competence protein ComER -
  EO946_RS12950 (EO946_12950) - 2515526..2516269 (-) 744 WP_003226141.1 class I SAM-dependent methyltransferase -
  EO946_RS12955 (EO946_12955) rsfS 2516266..2516622 (-) 357 WP_003226139.1 ribosome silencing factor -
  EO946_RS12960 (EO946_12960) yqeK 2516640..2517200 (-) 561 WP_003226137.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  EO946_RS12965 (EO946_12965) - 2517190..2517759 (-) 570 WP_003226135.1 nicotinate-nucleotide adenylyltransferase -
  EO946_RS12970 (EO946_12970) yhbY 2517771..2518061 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -

Sequence

Protein


Download         Length: 776 a.a.        Molecular weight: 86621.22 Da        Isoelectric Point: 7.9910

>NTDB_id=333593 EO946_RS12930 WP_003226149.1 2510968..2513298(-) (comEC) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
MRNSRLLLPLAAASATAGITAAAYFSAVFLFFLFLLIVLIKTRHAFLIFVCFFSFILFFSLYAVTDSQNVSLYQRGTYQF
KAVIDSIPKIDGDRMSMVVKTPDSEKWAASYRIQSADEKDRLSYIEPGMACELTGSLEEPKHSTVPGTFDYNEYLYQQHI
HWNYSVTSIQNCSEPANFMYKLLSLRKYIISFTNDLLPPNSAGIVQALTVGDRFNVEDEVLNAYQKIGVVHLLAISGLHI
GILTAGLFYIMIRIGITREKASIMLLLFLPLYVVLTGAAPSVLRAALMSGIYLAGSLVKWRVHSAGAVCLSYIVLLLFNP
YYLFEAGFQLSFAVSFSLILSSSIFKRVKTFLGQLAMVSMIAQLGSLPILLYHFQQFSIISIPMNMVLVPFYTFCVLPGA
IAGVFLLSLSVSAGRLFFGWFDFLMSWANKLITKIAEIDVFTIIIARPAPALLFLFIATIILLLMAIEKRSFSQLMVTGG
MCCAVLSLLFIYPRLSSEGEVDMIDIGQGDSMYVGAPHQRGHVLIDTGGTLPYLSEPWREKQHPFSLGEKVLIPFLTAKG
IKQLDALILTHADQDHIGEAETLLKHHKVKRLVIPKGFVSEPKDEKVLQAAREEGVPIEEVKRGDVLQIKDLQFHVLSPE
TPDPASKNNSSLVLWMKTGGMSWVLTGDLEKEGEQEVMNVFPNIKADVLKVGHHGSKGSTGEEFIKQLQPKTAIISAGIN
NRYHHPHQEVLQLLQRHSIRVLRTDQNGTIQYRYKNRVGTFSVYSPYDTSDIAETN

Nucleotide


Download         Length: 2331 bp        

>NTDB_id=333593 EO946_RS12930 WP_003226149.1 2510968..2513298(-) (comEC) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
ATGCGTAATTCGCGTTTGTTATTGCCTTTGGCGGCAGCTTCGGCAACTGCTGGAATTACTGCCGCCGCTTATTTCTCCGC
TGTTTTTCTCTTCTTCCTCTTTCTCCTCATCGTTTTAATCAAAACGAGACACGCTTTTCTCATTTTTGTTTGTTTCTTCT
CTTTTATATTGTTTTTTTCACTGTATGCAGTCACAGACTCCCAGAATGTCTCTTTATATCAGCGGGGCACCTATCAATTC
AAGGCAGTGATTGACAGTATTCCTAAAATTGATGGTGACCGTATGTCTATGGTAGTTAAGACGCCTGATAGTGAAAAATG
GGCCGCATCGTATCGCATTCAGTCTGCTGATGAAAAAGATCGGCTTTCATACATAGAACCAGGAATGGCATGTGAATTAA
CTGGTTCATTGGAAGAACCGAAACATTCAACTGTGCCGGGTACATTTGATTATAACGAATATCTTTACCAACAGCATATT
CACTGGAACTACTCTGTCACATCAATCCAAAATTGCAGCGAACCTGCAAATTTTATGTATAAGCTGCTCAGTTTGAGAAA
ATACATTATATCATTCACAAACGACCTGCTGCCTCCTAATTCGGCAGGAATCGTACAAGCACTTACAGTCGGTGACAGAT
TTAATGTGGAAGACGAAGTGCTTAACGCGTATCAAAAGATTGGTGTCGTCCATCTCTTGGCGATTTCAGGACTCCACATT
GGCATTTTGACAGCCGGCTTGTTTTATATCATGATCCGTATTGGTATAACAAGAGAAAAGGCGTCAATCATGTTGCTGTT
ATTTCTGCCGCTCTATGTGGTGTTGACGGGCGCTGCTCCTTCAGTGCTTCGCGCCGCTCTGATGTCGGGAATTTACTTAG
CAGGAAGTCTCGTGAAATGGCGTGTACATTCAGCCGGTGCTGTCTGCCTTTCATATATTGTCCTCTTGCTCTTCAATCCT
TACTATCTCTTCGAAGCCGGTTTTCAGCTGTCGTTCGCCGTCAGTTTTTCTTTAATACTTTCCTCTTCTATTTTTAAGCG
TGTCAAAACCTTCTTAGGTCAGCTGGCAATGGTATCGATGATCGCTCAGCTGGGCTCACTTCCTATTCTCCTGTATCATT
TCCAGCAGTTTTCTATAATTAGCATACCGATGAACATGGTGCTGGTTCCTTTTTATACATTCTGCGTTTTGCCGGGAGCT
ATAGCAGGTGTTTTTCTATTAAGCCTTTCCGTTTCTGCCGGTCGATTGTTTTTCGGCTGGTTTGATTTCTTGATGAGCTG
GGCCAACAAGCTCATCACAAAAATTGCAGAGATTGATGTATTCACGATCATCATTGCACGCCCCGCACCTGCTCTGCTCT
TTTTGTTCATAGCCACGATAATCCTATTGCTTATGGCGATTGAAAAACGCTCCTTTTCGCAGCTGATGGTAACTGGCGGC
ATGTGTTGCGCTGTGCTCAGTCTTCTCTTTATTTATCCTCGTCTTAGTTCTGAAGGAGAGGTCGATATGATTGATATTGG
GCAGGGTGACAGCATGTATGTAGGTGCTCCGCATCAGCGAGGCCATGTCTTAATTGATACTGGCGGCACTTTGCCTTACT
TGTCAGAGCCTTGGCGGGAAAAACAGCACCCGTTTTCACTGGGAGAAAAGGTGCTGATTCCGTTTTTAACTGCTAAGGGA
ATTAAACAGCTTGATGCTTTAATTCTGACGCACGCTGACCAAGATCATATTGGAGAGGCGGAGACTCTGCTGAAGCATCA
TAAAGTGAAGCGCCTAGTGATTCCGAAAGGGTTCGTTTCTGAACCTAAAGACGAAAAAGTGCTGCAGGCTGCCAGAGAGG
AGGGAGTGCCAATTGAAGAGGTGAAGCGAGGCGATGTATTGCAAATAAAGGACTTGCAGTTCCATGTACTGTCACCGGAA
ACACCTGACCCGGCAAGCAAAAATAATTCTTCTCTCGTTCTGTGGATGAAAACGGGCGGTATGAGCTGGGTATTGACGGG
GGATCTGGAGAAAGAAGGAGAACAGGAGGTAATGAACGTGTTTCCAAATATAAAAGCGGATGTATTAAAGGTGGGGCACC
ATGGAAGCAAAGGCTCTACCGGTGAAGAATTCATCAAACAGCTTCAGCCGAAAACAGCCATTATCTCGGCGGGGATAAAC
AATCGGTACCATCATCCCCATCAAGAAGTTCTGCAATTATTACAAAGACATTCCATCCGCGTGCTCCGCACCGATCAAAA
CGGAACAATCCAATATAGATATAAAAACAGAGTTGGAACCTTTTCTGTCTATTCTCCATATGATACATCAGATATAGCAG
AGACGAACTAA

Domains

Predicted by InterproScan.

Domain of unknown function (DUF4131) (DUF4131)

(20-170)

Metallo-beta-lactamase superfamily (Lactamase_B)

(507-718)

Competence protein (Competence)

(208-469)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEC Bacillus subtilis subsp. subtilis str. 168

88.144

100

0.881

Multiple sequence alignment