Detailed information    

insolico Bioinformatically predicted

Overview


Name   sinI   Type   Regulator
Locus tag   EO946_RS12425 Genome accession   NZ_CP034943
Coordinates   2426637..2426810 (+) Length   57 a.a.
NCBI ID   WP_003226347.1    Uniprot ID   G4NQ83
Organism   Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633     
Function   inhibit the expression of sinR (predicted from homology)   
Competence regulation

Genomic Context


Location: 2421637..2431810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EO946_RS12405 (EO946_12405) gcvT 2422432..2423520 (-) 1089 WP_003226353.1 glycine cleavage system aminomethyltransferase GcvT -
  EO946_RS12410 (EO946_12410) - 2423963..2425636 (+) 1674 WP_003226350.1 DEAD/DEAH box helicase -
  EO946_RS12415 (EO946_12415) - 2425657..2426451 (+) 795 WP_003226349.1 YqhG family protein -
  EO946_RS12425 (EO946_12425) sinI 2426637..2426810 (+) 174 WP_003226347.1 anti-repressor SinI Regulator
  EO946_RS12430 (EO946_12430) sinR 2426844..2427179 (+) 336 WP_003226345.1 transcriptional regulator SinR Regulator
  EO946_RS12435 (EO946_12435) tasA 2427273..2428058 (-) 786 WP_003226340.1 biofilm matrix protein TasA -
  EO946_RS12440 (EO946_12440) sipW 2428122..2428694 (-) 573 WP_003226338.1 signal peptidase I SipW -
  EO946_RS12445 (EO946_12445) tapA 2428678..2429439 (-) 762 WP_003226335.1 amyloid fiber anchoring/assembly protein TapA -
  EO946_RS12450 (EO946_12450) - 2429708..2430034 (+) 327 WP_079996407.1 YqzG/YhdC family protein -
  EO946_RS12455 (EO946_12455) - 2430076..2430255 (-) 180 WP_003226330.1 YqzE family protein -
  EO946_RS12460 (EO946_12460) comGG 2430327..2430701 (-) 375 WP_003226328.1 competence type IV pilus minor pilin ComGG Machinery gene
  EO946_RS12465 (EO946_12465) comGF 2430702..2431085 (-) 384 WP_032727308.1 competence type IV pilus minor pilin ComGF Machinery gene
  EO946_RS12470 (EO946_12470) comGE 2431111..2431458 (-) 348 WP_003226323.1 competence type IV pilus minor pilin ComGE Machinery gene

Sequence


Protein


Download         Length: 57 a.a.        Molecular weight: 6632.64 Da        Isoelectric Point: 8.6596

>NTDB_id=333583 EO946_RS12425 WP_003226347.1 2426637..2426810(+) (sinI) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
MKNAKQEHFELDQEWVELMMKAKEANISPEEIRKYLLLNKKSAHPGPAARSHTVNPF

Nucleotide


Download         Length: 174 bp        

>NTDB_id=333583 EO946_RS12425 WP_003226347.1 2426637..2426810(+) (sinI) [Bacillus spizizenii ATCC 6633 = JCM 2499 strain ATCC 6633]
ATGAAAAATGCAAAACAAGAGCACTTCGAATTAGATCAAGAATGGGTTGAATTAATGATGAAAGCCAAAGAGGCAAATAT
CAGCCCGGAAGAAATACGAAAATATTTACTTTTAAACAAAAAGTCTGCTCATCCTGGTCCGGCAGCCAGAAGTCATACCG
TAAATCCTTTCTGA

Domains


Predicted by InterproScan.

(11-36)


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB G4NQ83

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sinI Bacillus subtilis subsp. subtilis str. 168

96.491

100

0.965


Multiple sequence alignment