Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   EM867_RS23355 Genome accession   NZ_CP034908
Coordinates   5053425..5053874 (-) Length   149 a.a.
NCBI ID   WP_003112842.1    Uniprot ID   P04739
Organism   Pseudomonas aeruginosa strain PA0750     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5048425..5058874
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EM867_RS23335 (EM867_24585) ampD 5049117..5049683 (-) 567 WP_003112844.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  EM867_RS23340 (EM867_24590) - 5049834..5052089 (-) 2256 WP_003112843.1 DUF1631 domain-containing protein -
  EM867_RS23345 (EM867_24595) nadC 5052375..5053223 (+) 849 WP_003104915.1 carboxylating nicotinate-nucleotide diphosphorylase -
  EM867_RS23355 (EM867_24605) pilA 5053425..5053874 (-) 450 WP_003112842.1 type 4a pilus biogenesis protein PilA Machinery gene
  EM867_RS23360 (EM867_24610) pilB 5054106..5055806 (+) 1701 WP_003112841.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EM867_RS23365 (EM867_24615) pilC 5055810..5057034 (+) 1225 Protein_4632 type 4a pilus biogenesis protein PilC -
  EM867_RS23370 (EM867_24620) pilD 5057038..5057910 (+) 873 WP_003112839.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  EM867_RS23375 (EM867_24625) coaE 5057907..5058518 (+) 612 WP_003112838.1 dephospho-CoA kinase -
  EM867_RS23380 (EM867_24630) yacG 5058515..5058715 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15511.78 Da        Isoelectric Point: 6.2905

>NTDB_id=333434 EM867_RS23355 WP_003112842.1 5053425..5053874(-) (pilA) [Pseudomonas aeruginosa strain PA0750]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYV
GVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN

Nucleotide


Download         Length: 450 bp        

>NTDB_id=333434 EM867_RS23355 WP_003112842.1 5053425..5053874(-) (pilA) [Pseudomonas aeruginosa strain PA0750]
ATGAAAGCTCAAAAAGGCTTTACCTTGATCGAACTGATGATCGTGGTTGCGATCATCGGTATCCTGGCGGCAATTGCCAT
TCCCCAGTATCAGAACTATGTTGCGCGTTCGGAAGGTGCTTCGGCGCTGGCGACGATCAACCCGCTGAAGACCACTGTTG
AAGAGTCGCTGTCGCGTGGAATTGCTGGTAGCAAAATTAAAATTGGTACTACTGCTTCTACTGCGACCGAAACATATGTC
GGCGTCGAGCCGGATGCCAACAAGTTGGGTGTAATTGCTGTAGCAATCGAAGATAGTGGTGCGGGTGATATTACCTTTAC
CTTCCAGACTGGTACCTCTAGTCCCAAGAATGCTACTAAAGTTATCACTCTGAACCGTACTGCGGATGGGGTCTGGGCTT
GTAAATCTACCCAGGATCCGATGTTCACTCCGAAAGGTTGTGATAACTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 1PAN
  PDB 1PAO
  PDB 8TUM
  PDB 8TUW
  PDB 9EWX

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

67.785

100

0.678
  pilA Acinetobacter baumannii strain A118

48

100

0.483
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

48.322

100

0.483
  pilA Vibrio cholerae strain A1552

48.322

100

0.483
  pilA Vibrio cholerae C6706

48.322

100

0.483
  pilA/pilAI Pseudomonas stutzeri DSM 10701

45.638

100

0.456
  pilA Vibrio parahaemolyticus RIMD 2210633

44.898

98.658

0.443
  comP Acinetobacter baylyi ADP1

41.83

100

0.43
  pilA/pilAII Pseudomonas stutzeri DSM 10701

44.056

95.973

0.423
  pilA2 Legionella pneumophila str. Paris

38.776

98.658

0.383
  pilA2 Legionella pneumophila strain ERS1305867

38.095

98.658

0.376

Multiple sequence alignment