Detailed information NTG0333337

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: ENC22_RS07825
• Genome accession: NZ_CP034836
• Coordinates: 1735588..1736802 (-)
• Length: 404 a.a.
• NCBI ID: WP_127969228.1
• Uniprot ID: A0AAE5XHU2
• Organism: Hahella sp. KA22
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1730588..1741802

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ENC22_RS07800 (ENC22_07800)	-	1730771..1731433 (-)	663	WP_127969223.1	SDR family oxidoreductase	-
ENC22_RS07805 (ENC22_07805)	-	1731517..1732086 (-)	570	WP_127969224.1	NADPH-dependent FMN reductase	-
ENC22_RS07810 (ENC22_07810)	-	1732453..1733049 (+)	597	WP_127969225.1	DUF938 domain-containing protein	-
ENC22_RS07815 (ENC22_07815)	-	1733101..1733754 (-)	654	WP_127969226.1	DUF4291 domain-containing protein	-
ENC22_RS07820 (ENC22_07820)	-	1734022..1735398 (-)	1377	WP_127969227.1	wax ester/triacylglycerol synthase family O-acyltransferase	-
ENC22_RS07825 (ENC22_07825)	pilU	1735588..1736802 (-)	1215	WP_127969228.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
ENC22_RS07830 (ENC22_07830)	-	1737091..1739637 (+)	2547	WP_127969229.1	EAL domain-containing protein	-
ENC22_RS07835 (ENC22_07835)	-	1739691..1740515 (-)	825	WP_127969230.1	OmpA family protein	-
ENC22_RS07840 (ENC22_07840)	-	1740658..1741476 (-)	819	WP_127969231.1	alpha/beta fold hydrolase	-

Sequence

Protein

• Length: 404 a.a.
• Molecular weight: 44867.14 Da
• Isoelectric Point: 5.4889

>NTDB_id=333337 ENC22_RS07825 WP_127969228.1 1735588..1736802(-) (pilU) [Hahella sp. KA22]
MEFNQYLTVLAKNDGSDLYLSTGAPPCAKFHGKLKPIDKTPIPPGRIMEIANSIMDEEQREQFAHELEMNLAYSIPQVGR
FRVNIFKQRNEVSIVARNIVTDIPNADSLGLPPILKEVLMSKRGLILFVGATGSGKSTSLAALIDHRNSNSGGHIITIED
PVEFIHKHKKSIINQREVGVDTRSYHNALKNTLRQAPDVILIGEIRDRETMEHALAFAETGHLCISTLHANNSNQALDRI
INFFPEERRNQLLMDLSLNLRAFVSQRLVPTIDGKRTAAIEILLGTPTVSELILRGDIDGIKEIMQKSENIGMQTFDAAL
FKLVQQEKISVEEALKNADSANNLRLRIKLHAGVEVPTSLGDEKVEDAGPEELAAKKPSSGFSLSLQEIEEDDEEDNDPF
KPKS

Nucleotide

• Length: 1215 bp

>NTDB_id=333337 ENC22_RS07825 WP_127969228.1 1735588..1736802(-) (pilU) [Hahella sp. KA22]
ATGGAATTCAACCAGTATCTGACCGTTCTGGCCAAGAATGACGGGTCCGACCTCTACCTGAGCACCGGCGCGCCGCCCTG
CGCCAAGTTTCACGGTAAATTGAAGCCCATCGATAAGACCCCCATTCCGCCGGGCAGAATTATGGAAATCGCCAACTCCA
TCATGGATGAAGAACAGCGCGAGCAGTTCGCCCATGAGCTGGAAATGAATCTGGCGTACAGCATCCCTCAGGTTGGCCGT
TTCCGGGTCAACATCTTCAAACAGCGCAATGAAGTCTCCATCGTCGCCCGTAATATCGTGACCGATATTCCCAACGCTGA
CAGCCTGGGGCTGCCCCCTATCCTGAAAGAAGTATTGATGTCGAAACGGGGCTTGATTCTATTCGTCGGCGCTACCGGCT
CCGGCAAGTCCACCTCTCTGGCGGCGCTGATCGATCACCGCAACAGTAATAGCGGCGGTCATATCATTACTATCGAAGAC
CCGGTGGAGTTCATTCACAAGCACAAGAAAAGCATCATCAACCAGCGGGAAGTGGGCGTCGATACGCGCAGTTACCATAA
CGCGCTGAAAAACACCCTGCGGCAGGCGCCGGACGTCATTTTGATCGGCGAGATTCGCGACAGGGAAACCATGGAGCACG
CGCTGGCTTTCGCAGAAACCGGGCACCTGTGTATCTCCACGCTGCACGCCAACAACTCTAACCAGGCGCTGGACCGCATC
ATTAACTTTTTCCCGGAAGAACGCCGCAACCAGTTGCTGATGGATTTGTCGTTGAACCTGCGAGCCTTCGTCTCACAACG
TTTGGTGCCGACGATTGACGGTAAGCGCACCGCCGCCATCGAGATCCTGCTGGGTACGCCCACGGTCAGCGAATTGATTC
TGCGCGGCGACATCGACGGCATCAAAGAGATCATGCAGAAGTCGGAAAACATCGGCATGCAGACCTTTGACGCAGCGCTG
TTCAAGCTGGTGCAGCAGGAAAAAATCAGCGTGGAGGAAGCCCTGAAAAACGCCGACTCCGCCAACAACCTGCGTCTGCG
CATCAAACTGCACGCCGGGGTGGAAGTTCCCACCAGCCTGGGCGATGAAAAAGTTGAGGACGCCGGCCCCGAAGAACTGG
CGGCCAAGAAGCCCAGCAGCGGTTTTTCTCTCAGCCTGCAGGAAATTGAAGAGGACGACGAGGAGGACAACGACCCCTTC
AAACCTAAAAGTTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (114-269)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	57.835	86.881	0.502
pilU	Acinetobacter baylyi ADP1	56.534	87.129	0.493
pilU	Vibrio cholerae strain A1552	49.435	87.624	0.433
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.576	94.554	0.384