Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   CA694_RS03575 Genome accession   NZ_CP042973
Coordinates   747126..747785 (-) Length   219 a.a.
NCBI ID   WP_001221493.1    Uniprot ID   Q3YXL4
Organism   Escherichia coli strain CFSAN061768     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 742126..752785
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA694_RS03545 (CA694_003575) - 742242..743189 (+) 948 WP_001305988.1 iron-siderophore ABC transporter substrate-binding protein -
  CA694_RS03550 (CA694_003580) - 743186..744073 (+) 888 WP_000614953.1 MurR/RpiR family transcriptional regulator -
  CA694_RS03555 (CA694_003585) ygiN 744118..744432 (-) 315 WP_000958598.1 putative quinol monooxygenase -
  CA694_RS03560 (CA694_003590) mdaB 744463..745044 (-) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  CA694_RS03565 (CA694_003595) ygiZ 745402..745731 (+) 330 WP_001551659.1 DUF2645 family protein -
  CA694_RS03570 (CA694_003600) qseC 745780..747129 (-) 1350 WP_000673372.1 quorum sensing histidine kinase QseC -
  CA694_RS03575 (CA694_003605) ciaR 747126..747785 (-) 660 WP_001221493.1 quorum sensing response regulator transcription factor QseB Regulator
  CA694_RS03580 (CA694_003610) ygiW 747937..748329 (+) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  CA694_RS03585 (CA694_003615) ygiV 748382..748864 (+) 483 WP_000183492.1 GyrI-like domain-containing protein -
  CA694_RS03590 (CA694_003620) ygiS 748973..750580 (+) 1608 WP_001551657.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24687.63 Da        Isoelectric Point: 6.9850

>NTDB_id=333263 CA694_RS03575 WP_001221493.1 747126..747785(-) (ciaR) [Escherichia coli strain CFSAN061768]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=333263 CA694_RS03575 WP_001221493.1 747126..747785(-) (ciaR) [Escherichia coli strain CFSAN061768]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTATATAGTGCGCCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGTTAGCGGAACGTGTAGAGGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAACTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACTCTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGCAGTGATTTTATTCGTACCGTTCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)

Response regulator receiver domain (Response_reg)

(3-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q3YXL4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

38.326

100

0.397
  ciaR Streptococcus pneumoniae D39

38.326

100

0.397
  ciaR Streptococcus pneumoniae R6

38.326

100

0.397
  ciaR Streptococcus pneumoniae TIGR4

38.326

100

0.397
  ciaR Streptococcus mutans UA159

35.874

100

0.365

Multiple sequence alignment