Detailed information NTG0333134

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: FVF73_RS15500
• Genome accession: NZ_CP042934
• Coordinates: 3175194..3175853 (+)
• Length: 219 a.a.
• NCBI ID: WP_001221502.1
• Uniprot ID: B7LGG8
• Organism: Escherichia coli 042
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3170194..3180853

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FVF73_RS15475 (FVF73_15475)	ygiS	3171477..3173084 (-)	1608	WP_001375094.1	ABC transporter substrate-binding protein	-
FVF73_RS15480 (FVF73_15480)	mqsA	3173217..3173612 (-)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
FVF73_RS15485 (FVF73_15485)	mqsR	3173614..3173910 (-)	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	-
FVF73_RS15490 (FVF73_15490)	ygiV	3174115..3174597 (-)	483	WP_000183505.1	GyrI-like domain-containing protein	-
FVF73_RS15495 (FVF73_15495)	ygiW	3174650..3175042 (-)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
FVF73_RS15500 (FVF73_15500)	ciaR	3175194..3175853 (+)	660	WP_001221502.1	quorum sensing response regulator transcription factor QseB	Regulator
FVF73_RS15505 (FVF73_15505)	qseC	3175850..3177199 (+)	1350	WP_000673358.1	quorum sensing histidine kinase QseC	-
FVF73_RS15510 (FVF73_15510)	ygiZ	3177245..3177577 (-)	333	WP_000914690.1	DUF2645 family protein	-
FVF73_RS23995	-	3177584..3178294 (-)	711	WP_000834030.1	hypothetical protein	-
FVF73_RS24000	-	3178297..3178776 (-)	480	WP_000065332.1	Hcp family type VI secretion system effector	-
FVF73_RS15520 (FVF73_15520)	mdaB	3179026..3179607 (+)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
FVF73_RS15525 (FVF73_15525)	ygiN	3179638..3179952 (+)	315	WP_000958598.1	putative quinol monooxygenase	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24745.66 Da
• Isoelectric Point: 6.6543

>NTDB_id=333134 FVF73_RS15500 WP_001221502.1 3175194..3175853(+) (ciaR) [Escherichia coli 042]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALEERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=333134 FVF73_RS15500 WP_001221502.1 3175194..3175853(+) (ciaR) [Escherichia coli 042]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGAGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: B7LGG8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365