Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   FVE20_RS18795 Genome accession   NZ_CP042846
Coordinates   3868425..3869084 (+) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain JME64     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3863425..3874084
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FVE20_RS18770 ygiS 3864708..3866315 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  FVE20_RS18775 mqsA 3866448..3866843 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  FVE20_RS18780 mqsR 3866845..3867141 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  FVE20_RS18785 ygiV 3867346..3867828 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  FVE20_RS18790 ygiW 3867881..3868273 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  FVE20_RS18795 ciaR 3868425..3869084 (+) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  FVE20_RS18800 qseC 3869081..3870430 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  FVE20_RS18805 ygiZ 3870476..3870808 (-) 333 WP_000917684.1 DUF2645 family protein -
  FVE20_RS18810 mdaB 3871127..3871708 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  FVE20_RS18815 ygiN 3871739..3872053 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  FVE20_RS18820 parE 3872101..3873993 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=332267 FVE20_RS18795 WP_001221495.1 3868425..3869084(+) (ciaR) [Escherichia coli strain JME64]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=332267 FVE20_RS18795 WP_001221495.1 3868425..3869084(+) (ciaR) [Escherichia coli strain JME64]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361