Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   FVE21_RS18820 Genome accession   NZ_CP042845
Coordinates   3873306..3873965 (+) Length   219 a.a.
NCBI ID   WP_001221495.1    Uniprot ID   P52076
Organism   Escherichia coli strain JME65     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 3868306..3878965
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FVE21_RS18795 ygiS 3869589..3871196 (-) 1608 WP_001295629.1 ABC transporter substrate-binding protein -
  FVE21_RS18800 mqsA 3871329..3871724 (-) 396 WP_000650107.1 type II toxin-antitoxin system antitoxin MqsA -
  FVE21_RS18805 mqsR 3871726..3872022 (-) 297 WP_000415584.1 type II toxin-antitoxin system toxin MqsR -
  FVE21_RS18810 ygiV 3872227..3872709 (-) 483 WP_000183505.1 GyrI-like domain-containing protein -
  FVE21_RS18815 ygiW 3872762..3873154 (-) 393 WP_000712658.1 OB fold stress tolerance protein YgiW -
  FVE21_RS18820 ciaR 3873306..3873965 (+) 660 WP_001221495.1 quorum sensing response regulator transcription factor QseB Regulator
  FVE21_RS18825 qseC 3873962..3875311 (+) 1350 WP_000673402.1 quorum sensing histidine kinase QseC -
  FVE21_RS18830 ygiZ 3875357..3875689 (-) 333 WP_000917684.1 DUF2645 family protein -
  FVE21_RS18835 mdaB 3876008..3876589 (+) 582 WP_000065430.1 NADPH:quinone oxidoreductase MdaB -
  FVE21_RS18840 ygiN 3876620..3876934 (+) 315 WP_000958598.1 putative quinol monooxygenase -
  FVE21_RS18845 parE 3876982..3878874 (-) 1893 WP_000195296.1 DNA topoisomerase IV subunit B -

Sequence

Protein


Download         Length: 219 a.a.        Molecular weight: 24677.59 Da        Isoelectric Point: 6.9850

>NTDB_id=332185 FVE21_RS18820 WP_001221495.1 3873306..3873965(+) (ciaR) [Escherichia coli strain JME65]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLSRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide


Download         Length: 660 bp        

>NTDB_id=332185 FVE21_RS18820 WP_001221495.1 3873306..3873965(+) (ciaR) [Escherichia coli strain JME65]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAAAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGCGATGCGCTGGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAAGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGTCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGTT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(3-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(145-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P52076

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae Rx1

37.885

100

0.393
  ciaR Streptococcus pneumoniae D39

37.885

100

0.393
  ciaR Streptococcus pneumoniae R6

37.885

100

0.393
  ciaR Streptococcus pneumoniae TIGR4

37.885

100

0.393
  ciaR Streptococcus mutans UA159

35.426

100

0.361

Multiple sequence alignment