Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   SPJ_RS11060 Genome accession   NC_012466
Coordinates   2087745..2089043 (-) Length   432 a.a.
NCBI ID   WP_000867611.1    Uniprot ID   -
Organism   Streptococcus pneumoniae JJA     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2082745..2094043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SPJ_RS11040 (SPJ_2230) rplI 2083900..2084352 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  SPJ_RS11045 (SPJ_2231) - 2084349..2086322 (-) 1974 WP_000715111.1 DHH family phosphoesterase -
  SPJ_RS11050 (SPJ_2232) raiA 2086458..2087006 (-) 549 WP_000599107.1 ribosome-associated translation inhibitor RaiA -
  SPJ_RS11055 (SPJ_2233) comFC/cflB 2087086..2087748 (-) 663 WP_000649970.1 ComF family protein Machinery gene
  SPJ_RS11060 (SPJ_2234) comFA/cflA 2087745..2089043 (-) 1299 WP_000867611.1 DEAD/DEAH box helicase Machinery gene
  SPJ_RS11065 (SPJ_2235) - 2089099..2089734 (+) 636 WP_000395747.1 YigZ family protein -
  SPJ_RS11070 (SPJ_2236) cysK 2090019..2090939 (+) 921 WP_000029871.1 cysteine synthase A -
  SPJ_RS11075 (SPJ_2237) - 2091178..2091543 (-) 366 Protein_2085 IS66 family transposase -
  SPJ_RS11080 - 2091525..2091812 (+) 288 Protein_2086 transposase -
  SPJ_RS11085 (SPJ_2239) tnpB 2091967..2092221 (-) 255 WP_078373100.1 IS66 family insertion sequence element accessory protein TnpB -
  SPJ_RS11090 (SPJ_2240) tsf 2092536..2093576 (-) 1041 WP_000808063.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49590.50 Da        Isoelectric Point: 8.4382

>NTDB_id=33197 SPJ_RS11060 WP_000867611.1 2087745..2089043(-) (comFA/cflA) [Streptococcus pneumoniae JJA]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSNLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=33197 SPJ_RS11060 WP_000867611.1 2087745..2089043(-) (comFA/cflA) [Streptococcus pneumoniae JJA]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGACTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAATTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.074

100

0.991
  comFA/cflA Streptococcus pneumoniae D39

99.074

100

0.991
  comFA/cflA Streptococcus pneumoniae R6

99.074

100

0.991
  comFA/cflA Streptococcus pneumoniae TIGR4

98.843

100

0.988
  comFA/cflA Streptococcus mitis NCTC 12261

93.287

100

0.933
  comFA/cflA Streptococcus mitis SK321

92.13

100

0.921
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387

Multiple sequence alignment