Detailed information NTG0331673

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EJJ34_RS13955
• Genome accession: NZ_CP034484
• Coordinates: 2635740..2636357 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: A0AAE2SLR2
• Organism: Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2630740..2641357

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EJJ34_RS13925 (EJJ34_13930)	rpsT	2631042..2631308 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
EJJ34_RS13930 (EJJ34_13935)	holA	2631323..2632366 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
EJJ34_RS13935 (EJJ34_13940)	-	2632406..2632555 (-)	150	WP_003229985.1	hypothetical protein	-
EJJ34_RS13940 (EJJ34_13945)	yqzM	2632596..2632730 (+)	135	WP_003229983.1	YqzM family protein	-
EJJ34_RS13945 (EJJ34_13950)	comEC	2632770..2635100 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EJJ34_RS13950 (EJJ34_13955)	comEB	2635104..2635673 (-)	570	WP_003229978.1	ComE operon protein 2	-
EJJ34_RS13955 (EJJ34_13960)	comEA	2635740..2636357 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
EJJ34_RS13960 (EJJ34_13965)	comER	2636441..2637262 (+)	822	WP_004398597.1	late competence protein ComER	-
EJJ34_RS13965 (EJJ34_13970)	yqeM	2637328..2638071 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
EJJ34_RS13970 (EJJ34_13975)	rsfS	2638068..2638424 (-)	357	WP_003229971.1	ribosome silencing factor	-
EJJ34_RS13975 (EJJ34_13980)	yqeK	2638442..2639002 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EJJ34_RS13980 (EJJ34_13985)	nadD	2638992..2639561 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
EJJ34_RS13985 (EJJ34_13990)	yhbY	2639573..2639863 (-)	291	Protein_2690	ribosome assembly RNA-binding protein YhbY	-
EJJ34_RS13990 (EJJ34_13995)	aroE	2639857..2640699 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=331673 EJJ34_RS13955 WP_004398514.1 2635740..2636357(-) (comEA) [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=331673 EJJ34_RS13955 WP_004398514.1 2635740..2636357(-) (comEA) [Bacillus subtilis subsp. subtilis NCIB 3610 = ATCC 6051 = DSM 10 strain NCIB 3610]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39