Detailed information NTG0330963

Bioinformatically predicted

Overview

• Name: ciaR
• Type: Regulator
• Locus tag: FRV13_RS21385
• Genome accession: NZ_CP042298
• Coordinates: 3914061..3914720 (-)
• Length: 219 a.a.
• NCBI ID: WP_001221493.1
• Uniprot ID: Q3YXL4
• Organism: Escherichia coli strain RM9088
• Function: repress competence development; post-transcriptional repression of CSP production (predicted from homology) Competence regulation

Genomic Context

Location: 3909061..3919720

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FRV13_RS21360 (FRV13_21365)	ygiN	3909962..3910276 (-)	315	WP_000958598.1	putative quinol monooxygenase	-
FRV13_RS21365 (FRV13_21370)	mdaB	3910307..3910888 (-)	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
FRV13_RS28865	-	3911138..3911617 (+)	480	WP_000065333.1	Hcp family type VI secretion system effector	-
FRV13_RS28870	-	3911620..3912330 (+)	711	WP_000834029.1	hypothetical protein	-
FRV13_RS21375 (FRV13_21380)	ygiZ	3912337..3912669 (+)	333	WP_000914691.1	DUF2645 family protein	-
FRV13_RS21380 (FRV13_21385)	qseC	3912715..3914064 (-)	1350	WP_000673357.1	quorum sensing histidine kinase QseC	-
FRV13_RS21385 (FRV13_21390)	ciaR	3914061..3914720 (-)	660	WP_001221493.1	quorum sensing response regulator transcription factor QseB	Regulator
FRV13_RS21390 (FRV13_21395)	ygiW	3914872..3915264 (+)	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
FRV13_RS21395 (FRV13_21400)	ygiV	3915317..3915799 (+)	483	WP_000183505.1	GyrI-like domain-containing protein	-
FRV13_RS21400 (FRV13_21405)	mqsR	3916004..3916300 (+)	297	WP_000415585.1	type II toxin-antitoxin system toxin MqsR	-
FRV13_RS21405 (FRV13_21410)	mqsA	3916302..3916697 (+)	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	-
FRV13_RS21410 (FRV13_21415)	ygiS	3916830..3918437 (+)	1608	WP_001295629.1	ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 219 a.a.
• Molecular weight: 24687.63 Da
• Isoelectric Point: 6.9850

>NTDB_id=330963 FRV13_RS21385 WP_001221493.1 3914061..3914720(-) (ciaR) [Escherichia coli strain RM9088]
MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAPYDAVILDLTLPGMDGRDILREWREKGQREPVLILTA
RDALAERVEGLRLGADDYLCKPFALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGEK

Nucleotide

• Length: 660 bp

>NTDB_id=330963 FRV13_RS21385 WP_001221493.1 3914061..3914720(-) (ciaR) [Escherichia coli strain RM9088]
ATGCGAATTTTACTGATAGAAGATGACATGCTGATTGGCGACGGCATCAAAACGGGCCTTAGTAAAATGGGTTTTAGCGT
CGACTGGTTTACACAAGGTCGTCAGGGAAAAGAGGCGCTTTATAGCGCACCTTATGATGCGGTGATCCTGGATTTAACCT
TACCAGGCATGGATGGTCGCGATATTTTGCGCGAATGGCGAGAGAAAGGTCAGCGTGAGCCGGTACTGATCCTGACCGCG
CGTGATGCGTTAGCGGAACGTGTAGAAGGGCTGCGTCTGGGAGCTGACGATTATCTGTGTAAACCTTTTGCGTTGATAGA
AGTCGCCGCCAGGCTGGAGGCTCTGATGCGCCGAACCAACGGCCAGGCCAGCAACGAGCTGCGCCACGGTAACGTCATGC
TCGACCCCGGCAAACGTATCGCCACGCTGGCTGGCGAACCCTTAACACTGAAACCAAAAGAATTTGCCCTGCTGGAATTA
CTGATGCGTAACGCTGGTCGGGTACTGCCGCGCAAACTGATTGAAGAGAAACTGTATACCTGGGACGAAGAGGTCACCAG
TAATGCCGTTGAAGTGCATGTGCATCATCTGCGACGCAAACTCGGTAGTGATTTTATTCGTACCGTGCATGGTATTGGCT
ACACATTAGGTGAGAAATGA

Domains

Predicted by InterProScan.

• Transcriptional regulatory protein, C terminal (Trans_reg_C) (145-216)

• Response regulator receiver domain (Response_reg) (3-112)

3D structure

• AlphaFold DB: Q3YXL4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
ciaR	Streptococcus pneumoniae Rx1	38.326	100	0.397
ciaR	Streptococcus pneumoniae D39	38.326	100	0.397
ciaR	Streptococcus pneumoniae R6	38.326	100	0.397
ciaR	Streptococcus pneumoniae TIGR4	38.326	100	0.397
ciaR	Streptococcus mutans UA159	35.874	100	0.365