Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   SCA_RS00945 Genome accession   NC_012121
Coordinates   191563..192933 (+) Length   456 a.a.
NCBI ID   WP_012664210.1    Uniprot ID   B9DKY6
Organism   Staphylococcus carnosus subsp. carnosus TM300     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 186563..197933
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SCA_RS00925 (SCA_0178) - 186810..187271 (+) 462 WP_012664206.1 CtsR family transcriptional regulator -
  SCA_RS00930 (SCA_0179) - 187428..187814 (+) 387 Protein_175 UvrB/UvrC motif-containing protein -
  SCA_RS00935 (SCA_0180) - 187819..188823 (+) 1005 WP_012664208.1 protein arginine kinase -
  SCA_RS00940 (SCA_0181) - 188835..191300 (+) 2466 WP_012664209.1 ATP-dependent Clp protease ATP-binding subunit -
  SCA_RS00945 (SCA_0182) radA 191563..192933 (+) 1371 WP_012664210.1 DNA repair protein RadA Machinery gene
  SCA_RS00950 (SCA_0183) - 192964..194022 (+) 1059 WP_012664211.1 PIN/TRAM domain-containing protein -
  SCA_RS00955 (SCA_0184) gltX 194316..195770 (+) 1455 WP_012664212.1 glutamate--tRNA ligase -
  SCA_RS00960 (SCA_0185) cysE 196073..196702 (+) 630 WP_174221515.1 serine O-acetyltransferase -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 50255.84 Da        Isoelectric Point: 7.4017

>NTDB_id=33015 SCA_RS00945 WP_012664210.1 191563..192933(+) (radA) [Staphylococcus carnosus subsp. carnosus TM300]
MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIIEKKEAPSGRGMRTREQTAKVTKLNQVQNETTPRIKTSSPEFDR
VLGGGIVQGSLVLIGGDPGIGKSTLLLQICSALSQNKKVLYITGEESLNQTKLRADRLDEDSSNLNVFAETDLEVIKEAV
KQTEPDLVVVDSIQTIYHPDINSAPGSVSQVRESTQILMGIAKQMNIATFIVGHVTKEGQIAGPRLLEHMVDTVLYFEGD
EHHAYRILRAVKNRFGSTNEMGIFEMKHSGLKGVKNPSEMFLEERSTNVAGSTIVPTMEGTRPLLIEVQALVTPTTFNNP
RRMATGIDHNRLNLLMAVLEKKESYLLQQQDAYIKVAGGVRLTEPAVDLALIAAIASSFKDQPVNGMDCYVGEVGLTGEV
RRVSRIEQRVQEAAKLGFKRVIIPKTNSGGWDFPEGIQVIGVTSVHEALDFALMKR

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=33015 SCA_RS00945 WP_012664210.1 191563..192933(+) (radA) [Staphylococcus carnosus subsp. carnosus TM300]
TTGGCAAAAAAGAAAGTTATTTTTGAATGTATGGCATGTGGCTATCAATCCCCTAAATGGATGGGAAAATGTCCTAATTG
TGGTGCTTGGAACCAAATGGAAGAAATTATTGAGAAAAAAGAAGCGCCAAGTGGAAGAGGCATGCGTACACGAGAACAAA
CAGCAAAAGTTACCAAGCTTAACCAAGTACAAAATGAAACTACACCTCGTATCAAAACGAGTTCCCCTGAATTCGATAGA
GTATTAGGTGGCGGTATTGTACAAGGTTCGCTTGTCTTAATTGGCGGTGATCCTGGTATTGGTAAATCAACATTGCTGTT
GCAAATCTGTTCAGCATTATCACAAAATAAAAAAGTTTTATATATAACAGGTGAGGAATCACTGAACCAAACTAAATTAC
GAGCAGACCGTTTAGATGAAGATTCAAGTAATTTGAATGTGTTTGCAGAAACGGATTTAGAAGTGATTAAAGAAGCGGTC
AAACAGACTGAACCTGATTTAGTCGTAGTAGATTCTATTCAAACAATTTATCATCCGGATATCAACTCTGCGCCAGGTTC
TGTATCGCAAGTACGAGAAAGTACGCAAATTTTAATGGGTATTGCTAAACAAATGAATATTGCGACTTTCATTGTCGGTC
ACGTGACTAAAGAAGGTCAGATTGCAGGTCCGAGATTACTAGAGCACATGGTAGATACTGTATTGTATTTCGAAGGTGAC
GAACATCATGCGTACCGTATTTTACGTGCAGTTAAAAACCGTTTTGGTTCTACCAATGAAATGGGTATTTTCGAAATGAA
ACATAGCGGTTTGAAAGGTGTTAAAAACCCATCGGAAATGTTTTTAGAAGAACGGTCTACTAATGTGGCAGGTTCTACAA
TTGTGCCGACCATGGAAGGTACACGTCCGTTATTGATTGAAGTACAAGCATTAGTAACACCTACAACTTTCAATAACCCG
AGACGAATGGCAACGGGTATTGATCATAACCGTTTAAATTTATTGATGGCTGTACTTGAGAAAAAAGAAAGTTACTTACT
GCAGCAGCAAGATGCTTATATCAAAGTAGCAGGCGGAGTGAGACTGACTGAACCAGCTGTTGACTTAGCCCTGATTGCAG
CAATCGCTTCCAGCTTTAAAGACCAACCCGTAAATGGCATGGATTGTTATGTGGGTGAGGTTGGTTTAACAGGTGAAGTG
CGTCGCGTTTCACGTATCGAACAGCGTGTACAAGAAGCCGCAAAACTTGGATTTAAACGTGTGATTATCCCTAAAACCAA
TAGCGGTGGTTGGGATTTTCCTGAGGGTATTCAAGTAATTGGTGTAACGAGCGTGCACGAAGCGCTGGATTTCGCATTAA
TGAAACGCTGA

Domains

Predicted by InterproScan.

Lon protease (S16) C-terminal proteolytic domain (Lon_C)

(346-453)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(66-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B9DKY6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

66.228

100

0.662
  radA Streptococcus pneumoniae Rx1

60.579

98.465

0.596
  radA Streptococcus pneumoniae D39

60.579

98.465

0.596
  radA Streptococcus pneumoniae R6

60.579

98.465

0.596
  radA Streptococcus pneumoniae TIGR4

60.579

98.465

0.596
  radA Streptococcus mitis NCTC 12261

60.579

98.465

0.596
  radA Streptococcus mitis SK321

60.579

98.465

0.596

Multiple sequence alignment