Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   EHS18_RS07645 Genome accession   NZ_CP034098
Coordinates   1560381..1560878 (-) Length   165 a.a.
NCBI ID   WP_001794328.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 80wphwpl_v1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1555381..1565878
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EHS18_RS07620 (EHS18_07620) gcvPB 1555543..1557015 (-) 1473 WP_000202178.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  EHS18_RS07625 (EHS18_07625) gcvPA 1557008..1558354 (-) 1347 WP_000019697.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  EHS18_RS07630 (EHS18_07630) gcvT 1558374..1559465 (-) 1092 WP_000093347.1 glycine cleavage system aminomethyltransferase GcvT -
  EHS18_RS07635 (EHS18_07635) - 1559624..1560148 (-) 525 WP_001015125.1 shikimate kinase -
  EHS18_RS07640 (EHS18_07640) - 1560138..1560284 (-) 147 WP_001789879.1 hypothetical protein -
  EHS18_RS07645 (EHS18_07645) comGF 1560381..1560878 (-) 498 WP_001794328.1 competence type IV pilus minor pilin ComGF Machinery gene
  EHS18_RS07650 (EHS18_07650) comGE 1560796..1561095 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  EHS18_RS07655 (EHS18_07655) comGD 1561082..1561528 (-) 447 WP_001791207.1 competence type IV pilus minor pilin ComGD Machinery gene
  EHS18_RS07660 (EHS18_07660) comGC 1561506..1561817 (-) 312 WP_000472257.1 competence type IV pilus major pilin ComGC Machinery gene
  EHS18_RS07665 (EHS18_07665) comGB 1561831..1562901 (-) 1071 WP_000776412.1 competence type IV pilus assembly protein ComGB Machinery gene
  EHS18_RS07670 (EHS18_07670) comGA 1562873..1563847 (-) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  EHS18_RS07675 (EHS18_07675) - 1563899..1564522 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  EHS18_RS07680 (EHS18_07680) - 1564519..1564848 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  EHS18_RS07685 (EHS18_07685) - 1564848..1565834 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19197.95 Da        Isoelectric Point: 10.2192

>NTDB_id=328928 EHS18_RS07645 WP_001794328.1 1560381..1560878(-) (comGF) [Staphylococcus aureus strain 80wphwpl_v1]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=328928 EHS18_RS07645 WP_001794328.1 1560381..1560878(-) (comGF) [Staphylococcus aureus strain 80wphwpl_v1]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTAATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACAATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(68-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

97.576

100

0.976

Multiple sequence alignment