Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   EH233_RS25185 Genome accession   NZ_CP034058
Coordinates   6008367..6009476 (-) Length   369 a.a.
NCBI ID   WP_011320390.1    Uniprot ID   -
Organism   Anabaena sp. YBS01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6003367..6014476
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EH233_RS25165 (EH233_25055) - 6003726..6004052 (+) 327 WP_011320394.1 helix-turn-helix domain-containing protein -
  EH233_RS25170 (EH233_25060) - 6004186..6006621 (-) 2436 WP_011320393.1 type IV pilus secretin family protein -
  EH233_RS25175 (EH233_25065) - 6006739..6007500 (-) 762 WP_011320392.1 pilus assembly protein PilO -
  EH233_RS25180 (EH233_25070) - 6007497..6008264 (-) 768 WP_011320391.1 PilN domain-containing protein -
  EH233_RS25185 (EH233_25075) pilM 6008367..6009476 (-) 1110 WP_011320390.1 type IV pilus assembly protein PilM Machinery gene
  EH233_RS25190 (EH233_25080) - 6009709..6011007 (+) 1299 WP_011320389.1 sugar ABC transporter substrate-binding protein -
  EH233_RS25195 (EH233_25085) - 6011274..6012062 (-) 789 WP_011320388.1 FkbM family methyltransferase -
  EH233_RS25200 (EH233_25090) - 6012465..6013715 (-) 1251 WP_011320387.1 folylpolyglutamate synthase/dihydrofolate synthase family protein -
  EH233_RS25205 (EH233_25095) - 6013847..6013972 (-) 126 WP_010995199.1 photosystem II protein Y -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 40679.59 Da        Isoelectric Point: 4.5055

>NTDB_id=328643 EH233_RS25185 WP_011320390.1 6008367..6009476(-) (pilM) [Anabaena sp. YBS01]
MVKSFSSLFGKSHRGVGVEIAPERVNVVQIRKQRQGLKLESLTSVPVPEGIVTDGQITDPPAMAQLIQQALAESKIKKVS
RVATGVPGRDSIVRIIPVPSELDDKELRDMVLNHEAGLYLPYPREEADVDYQKLGYFVDEDGIEKVRVLLVATRKEVTDT
YISTFEQAGLQIGVLEINSFALIRTIRDQLRQFGPEEATVLVDIEFDSTEIAIIINGVPQFSRTVPIGTYQMQMAFARAM
SLPTSRDMEMLQGLVIPVNSVESDKTGMTETDPGIAGVLRILGELTDELRRSIDFYLNQSENVEIAQILLGGPGGGLEQL
HEFFTQRLSIPTSQIDPIGSLSLQVDEEKYPSTQRSGLGIVLGLGMREV

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=328643 EH233_RS25185 WP_011320390.1 6008367..6009476(-) (pilM) [Anabaena sp. YBS01]
GTGGTGAAAAGTTTCAGTAGTCTGTTTGGCAAATCTCATAGAGGGGTGGGTGTAGAAATTGCACCAGAACGGGTGAATGT
AGTTCAAATACGCAAGCAGCGCCAAGGCTTGAAACTTGAATCTTTAACATCAGTTCCAGTTCCGGAGGGGATTGTCACTG
ATGGTCAAATCACCGATCCTCCAGCGATGGCGCAATTAATTCAGCAGGCGCTGGCTGAAAGCAAAATCAAAAAAGTATCT
CGTGTGGCTACTGGTGTACCCGGACGAGATTCCATTGTCCGCATCATACCCGTACCATCAGAGTTGGATGACAAAGAATT
ACGGGATATGGTGTTAAATCACGAAGCCGGGTTGTATTTACCCTATCCTCGTGAAGAAGCTGATGTAGATTATCAGAAAC
TTGGGTACTTTGTGGATGAGGATGGCATTGAAAAAGTACGGGTGCTGTTAGTTGCTACCCGCAAGGAGGTGACAGATACA
TATATTAGTACTTTTGAGCAGGCAGGCTTACAAATTGGTGTTTTAGAAATTAACAGTTTTGCACTAATTCGGACAATTCG
TGATCAACTGCGGCAATTTGGGCCAGAAGAAGCAACGGTACTAGTTGATATAGAGTTCGACAGTACAGAAATTGCGATCA
TCATTAATGGCGTACCACAATTTTCCCGTACTGTCCCCATTGGCACTTATCAAATGCAAATGGCATTTGCTAGAGCTATG
AGTTTGCCCACTTCCAGAGATATGGAAATGTTACAGGGATTGGTGATTCCTGTAAATTCCGTAGAGAGCGATAAAACGGG
AATGACGGAAACCGACCCCGGTATTGCTGGCGTATTGCGAATCCTGGGAGAACTAACAGATGAGCTACGCCGTTCTATTG
ATTTTTACTTAAATCAGAGTGAAAACGTAGAAATAGCCCAGATTTTATTGGGTGGGCCTGGTGGCGGACTAGAACAACTT
CATGAGTTTTTTACTCAGCGTTTAAGTATTCCTACCAGTCAAATCGATCCTATAGGGTCTTTGTCCTTGCAAGTTGATGA
AGAAAAATATCCCTCTACACAACGTTCTGGCTTAGGAATAGTCCTTGGTTTAGGAATGAGGGAGGTATGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(284-368)

Type IV pilus assembly protein PilM; (PilM_2)

(17-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Synechocystis sp. PCC 6803

63.587

99.729

0.634

Multiple sequence alignment