Detailed information NTG0327469

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: EG882_RS10915
• Genome accession: NZ_CP033967
• Coordinates: 2072772..2073386 (+)
• Length: 204 a.a.
• NCBI ID: WP_015240245.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain 1B-23
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2067772..2078386

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EG882_RS10880 (EG882_10880)	aroE	2068459..2069301 (+)	843	WP_015240248.1	shikimate dehydrogenase	-
EG882_RS10885 (EG882_10885)	yhbY	2069295..2069585 (+)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
EG882_RS10890 (EG882_10890)	-	2069596..2070165 (+)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
EG882_RS10895 (EG882_10895)	yqeK	2070155..2070715 (+)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
EG882_RS10900 (EG882_10900)	rsfS	2070733..2071089 (+)	357	WP_007408260.1	ribosome silencing factor	-
EG882_RS10905 (EG882_10905)	-	2071086..2071823 (+)	738	WP_015240247.1	class I SAM-dependent methyltransferase	-
EG882_RS10910 (EG882_10910)	comER	2071892..2072713 (-)	822	WP_015240246.1	late competence protein ComER	-
EG882_RS10915 (EG882_10915)	comEA	2072772..2073386 (+)	615	WP_015240245.1	helix-hairpin-helix domain-containing protein	Machinery gene
EG882_RS10920 (EG882_10920)	-	2073453..2074022 (+)	570	WP_003152868.1	ComE operon protein 2	-
EG882_RS10925 (EG882_10925)	comEC	2074023..2076374 (+)	2352	WP_015240244.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
EG882_RS10930 (EG882_10930)	-	2076393..2076527 (-)	135	WP_003152870.1	YqzM family protein	-
EG882_RS10935 (EG882_10935)	-	2076568..2076719 (+)	152	Protein_2069	hypothetical protein	-
EG882_RS10940 (EG882_10940)	holA	2076759..2077800 (+)	1042	Protein_2070	DNA polymerase III subunit delta	-
EG882_RS10945 (EG882_10945)	rpsT	2077817..2078083 (-)	267	WP_003152876.1	30S ribosomal protein S20	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22209.32 Da
• Isoelectric Point: 5.5211

>NTDB_id=327469 EG882_RS10915 WP_015240245.1 2072772..2073386(+) (comEA) [Bacillus velezensis strain 1B-23]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=327469 EG882_RS10915 WP_015240245.1 2072772..2073386(+) (comEA) [Bacillus velezensis strain 1B-23]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCTGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACAGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGGAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGCACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCAATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAGAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363