Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   EGY13_RS03010 Genome accession   NZ_CP033858
Coordinates   520353..521093 (+) Length   246 a.a.
NCBI ID   WP_000076102.1    Uniprot ID   A0A1E3MBI9
Organism   Acinetobacter sp. FDAARGOS_493     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 515353..526093
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGY13_RS02995 (EGY13_02995) ponA 515940..518495 (-) 2556 WP_000736681.1 penicillin-binding protein PBP1a -
  EGY13_RS03000 (EGY13_03000) pilM 518657..519715 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  EGY13_RS03005 (EGY13_03005) pilN 519715..520356 (+) 642 WP_000201223.1 PilN domain-containing protein Machinery gene
  EGY13_RS03010 (EGY13_03010) pilO 520353..521093 (+) 741 WP_000076102.1 type 4a pilus biogenesis protein PilO Machinery gene
  EGY13_RS03015 (EGY13_03015) pilP 521104..521631 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  EGY13_RS03020 (EGY13_03020) pilQ 521693..523858 (+) 2166 WP_001017033.1 type IV pilus secretin PilQ family protein Machinery gene
  EGY13_RS03025 (EGY13_03025) aroK 523870..524439 (+) 570 WP_000643712.1 shikimate kinase AroK -
  EGY13_RS03030 (EGY13_03030) aroB 524468..525550 (+) 1083 WP_001193997.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27849.61 Da        Isoelectric Point: 4.8357

>NTDB_id=326442 EGY13_RS03010 WP_000076102.1 520353..521093(+) (pilO) [Acinetobacter sp. FDAARGOS_493]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGTTDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=326442 EGY13_RS03010 WP_000076102.1 520353..521093(+) (pilO) [Acinetobacter sp. FDAARGOS_493]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTATGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
TATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGACAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCCTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E3MBI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.593

100

0.996
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment