Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EGY23_RS03500 Genome accession   NZ_CP033832
Coordinates   748881..751025 (-) Length   714 a.a.
NCBI ID   WP_023083249.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain FDAARGOS_505     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 743881..756025
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EGY23_RS03485 (EGY23_03475) - 745527..747188 (-) 1662 WP_023083248.1 AAA family ATPase -
  EGY23_RS03490 (EGY23_03480) aroB 747195..748301 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  EGY23_RS03495 (EGY23_03485) aroK 748351..748869 (-) 519 WP_123789195.1 shikimate kinase AroK -
  EGY23_RS03500 (EGY23_03490) pilQ 748881..751025 (-) 2145 WP_023083249.1 type 4a pilus secretin PilQ Machinery gene
  EGY23_RS03505 (EGY23_03495) pilP 751079..751603 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  EGY23_RS03510 (EGY23_03500) pilO 751600..752223 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  EGY23_RS03515 (EGY23_03505) pilN 752220..752816 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  EGY23_RS03520 (EGY23_03510) pilM 752816..753880 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77402.02 Da        Isoelectric Point: 5.3214

>NTDB_id=326236 EGY23_RS03500 WP_023083249.1 748881..751025(-) (pilQ) [Pseudomonas aeruginosa strain FDAARGOS_505]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASAAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTMEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
RGYGKDGNIGIKDEDGMNCGPIAGNCTFPTTGTSKSPSPFVDIGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFDRALNGVPPINKN
EVNAKILVNDGETIVIGGVFSNTQSKSVDKVPFLGDLPYLGRLFRRDTVSDVKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=326236 EGY23_RS03500 WP_023083249.1 748881..751025(-) (pilQ) [Pseudomonas aeruginosa strain FDAARGOS_505]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCGTTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGAGACCGGGTCGAGCTAAAGTTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAG
TTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACGCGGTTGATCATCAACCTGACCACGCT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCTGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATCCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATTCAAGAACAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAACCGATGACCATGGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCTGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTCCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGTCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAACTGG
AGAGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGCAACTGTAC
TTTCCCCACCACGGGAACCAGCAAGTCGCCGTCGCCGTTCGTCGACATCGGCGCCAAGGATGCCACCTCCGGAATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCCAGGAAGTACCCTACCAGGAGGCCAGCTC
CAGTGGCGCTACCAGCACCTCGTTCAAGGAGGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCAGACAACCGGA
TCATCGTCGAAGTGAAGGTGACCAAGGATGCGCCGGACTTCGATCGCGCACTGAACGGCGTACCGCCGATCAACAAGAAT
GAGGTGAACGCCAAGATCCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGCAACACCCAGAGCAAGTC
GGTGGACAAGGTGCCGTTCCTCGGCGACCTGCCTTACCTGGGGCGGCTGTTCCGGCGCGATACGGTCTCGGACGTGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-122)

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

Bacterial type II and III secretion system protein (Secretin)

(549-705)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

97.899

100

0.979
  pilQ Acinetobacter baumannii strain A118

41.268

99.44

0.41
  pilQ Acinetobacter baumannii D1279779

41.268

99.44

0.41
  comQ Acinetobacter baylyi ADP1

41.536

98.459

0.409

Multiple sequence alignment