Detailed information NTG0324776

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: EGV94_RS26325
• Genome accession: NZ_CP033685
• Coordinates: 5508634..5509971 (+)
• Length: 445 a.a.
• NCBI ID: WP_003094694.1
• Uniprot ID: A0A0H2ZG94
• Organism: Pseudomonas aeruginosa strain H26023
• Function: regulate pilin expression (predicted from homology) Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5510017..5518141	5508634..5509971	flank	46

Gene organization within MGE regions

Location: 5508634..5518141

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
EGV94_RS26325 (EGV94_26270)	pilR	5508634..5509971 (+)	1338	WP_003094694.1	two-component system response regulator PilR	Regulator
EGV94_RS26330 (EGV94_26275)	thiO	5510017..5511111 (-)	1095	WP_003141551.1	glycine oxidase ThiO	-
EGV94_RS26335 (EGV94_26280)	-	5511251..5511751 (+)	501	WP_016254062.1	GspH/FimT family pseudopilin	-
EGV94_RS26340 (EGV94_26285)	-	5511874..5512362 (+)	489	WP_003141552.1	GspH/FimT family pseudopilin	-
EGV94_RS26345 (EGV94_26290)	pilV	5512372..5512896 (+)	525	WP_003116261.1	type IV pilus modification protein PilV	Machinery gene
EGV94_RS26350 (EGV94_26295)	-	5512905..5513720 (+)	816	WP_016254061.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
EGV94_RS26355 (EGV94_26300)	-	5513717..5514322 (+)	606	WP_003116263.1	pilus assembly protein	-
EGV94_RS26360 (EGV94_26305)	-	5514336..5517812 (+)	3477	WP_003141555.1	type IV pilus biogenesis factor PilY1	-
EGV94_RS26365 (EGV94_26310)	-	5517815..5518141 (+)	327	WP_016254060.1	PilY2 family type 4a fimbrial biogenesis protein	-

Sequence

Protein

• Length: 445 a.a.
• Molecular weight: 49737.06 Da
• Isoelectric Point: 5.8654

>NTDB_id=324776 EGV94_RS26325 WP_003094694.1 5508634..5509971(+) (pilR) [Pseudomonas aeruginosa strain H26023]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDLCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide

• Length: 1338 bp

>NTDB_id=324776 EGV94_RS26325 WP_003094694.1 5508634..5509971(+) (pilR) [Pseudomonas aeruginosa strain H26023]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACCTGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTACGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGCTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAGCTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGTGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCACCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTATACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATCGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCACTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCCCAGCG
CCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

• Response regulator receiver domain (Response_reg) (7-115)

• Sigma-54 interaction domain (Sigma54_activat) (135-301)

• Bacterial regulatory protein, Fis family (HTH_8) (402-442)

3D structure

• AlphaFold DB: A0A0H2ZG94

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	100	100	1
pilR	Acinetobacter baumannii strain A118	51.198	100	0.528
luxO	Vibrio cholerae strain A1552	38.17	100	0.384